犬肝硬化门静脉高压征食管静脉曲张模型的建立和评价.docVIP

犬肝硬化门静脉高压征食管静脉曲张模型的建立及评价 　　作者：王艳梅，李楠，王雅文，王欣，张林 作者单位：(解放军总医院第二附属医院 消化科，北京 100091) 　　【摘要】目的 食管胃底静脉曲张动物模型的建立，对研究如何预防和治疗门脉高压征食管胃底静脉曲张出血具有重要意义。本研究目的在于建立犬门脉高压征食管静脉曲张动物模型并加以评价，为下一步探索临床治疗方法提供可靠、稳定的模型动物。方法 10条健康成年雄性杂种犬为研究对象，以胆总管结扎术加CCl4法建立门脉高压征食管胃底静脉曲张动物模型。同时采用一系列的检查方法加以评估。结果 10条犬存活8条，经胃镜检查证实，所有存活动物均形成食管静脉曲张，其中轻度2例，中度4例，重度2例。与术前相比，肝功能显著异常，门静脉压力明显升高，差异具有显著统计学意义(P0.01)。门脉高压性胃病达58%。 结论 本组方法简单易行，模型质量可靠，制备周期明显缩短。该方法可形成稳定的肝硬化门脉高压征食管静脉曲张模型，可用于该方面的相关研究。 　　【关键词】 犬;动物模型;食管静脉曲张;门脉高压征 　　Establishment and Evaluation of Esophageal Varices in Dogs with Portal Hypertension 　　Wang Yanmei, Li Nan, Wang Yawen, Wang Xin, Zhang Lin 　　(Department of Gastroenterology, Second Affiliated Hospital of PLA General Hospital, Beijing 100091, China) 　　Abstract： Objective To develop and evaluate the canine model of esophageal varices with portal hypertension for successive study on the treatment of bleeding caused by esophageal varices.Methods Ten healthy adult mongrel dogs were used in the study. Esophageal varices were established by complete ligation of common bile duct and injection of CCL4.Results After operation, 8 in 10 dogs survived for six to eight weeks. All the dogs who survived had developed esophageal varices that were demonstrated by endoscopy, i.e. mild in two, middle in four, and severe in two. Their liver function was abnormal, and the average portal pressure was significantly higher than that of controls. The number of veins and the vessel area in esophageal submucosa of the trial dogs increased significantly, compared with that of the controls (P0.01).Conclusion The procedure is fairly stable and reliable, the model is useable for research in esophageal varices. Compared with other methods, our procedure is simpler and easier, and the period of production is much shorter. Furthermore, the model quality is reliable and stability. 　　Key words： canine; animal model; esophageal varices; portal hypertension 　　由于病毒性肝炎、酒精性肝病等原因造成的慢性肝病和肝硬化逐渐增多，肝硬化一直是消化学界的研究热点。其