小鼠同种异基因骨髓移植aGVHD动物模型建立.docVIP

小鼠同种异基因骨髓移植aGVHD动物模型的建立 作者 作者单位 蔡芳芳 温州医学院附属第一医院 血液科，浙江 温州 32500 俞康 温州医学院附属第一医院 血液科，浙江 温州 32500 江松福 温州医学院附属第一医院 血液科，浙江 温州 325000 Establishment of a mouse model of acute graft-versus-host disease after allogeneic bone marrow transplantation CAI Fang-fang，YU Kang，JIANG Song-fu. Department of Hematology，the First Af-filiated Hospital of Wenzhou Medical College，Wenzhou，325000 Abstract: Objective: To establish a mouse model of acute graft-versus-host disease (aGVHD) after allogeneic bone marrow transplantation (Allo-BMT) for providing experimental studies of aGVHD. Methods：Male C57BL/6(H-2b)mice were used as donors and female BALB/C(H-2d)mice as recipients.All BALB/C(H-2d) mice were randomly divided into 7 groups and received total body irradiation (TBI) 8.0Gy at 4～6 hours prior to transplantation.Bone marrow cells mixed with spleen cells of donors based upon different proportions were injected into the tail vein.Their survival time，leucocyte counts of peripheral blood，clinical and pathologic manifestations of aGVHD were evaluated.Results：Several mice in the group infused with only bone marrow cells showed insignificant aGVHD evidence，75% mice survived for a long period (30 d). There were significant differences in the incidence and severity of aGVHD with different ratios of bone marrow cells and spleen cells. The groups with co-transplantation of bone marrow cells and spleen cells in ratio of 1.5:1 or 1:1 showed typical clinical and pathological manifestations of aGVHD at a relatively concentrated time(8～15 d).Conclusion ：To establish a mice model of aGVHD needs additional donor’s spleen cells. Infusion of bone marrow cells and spleen cells in ratio of 1.5:1 or 1:1 is perfect as to establish aGVHD mouse model after allogeneic bone marrow transplantation. Key words: mice;allogeneic bone marrow transplantation;acute graft-versus-host disease; animal model 迄今为止，移植物抗宿主病(graft versus host disease，GVHD)仍是影响异基因骨髓移植(allog