环氧合酶1是化学肝损伤重要保护因子.pdfVIP

环氧合酶 1 是化学肝损伤的重要保护因子 1,2 1 2 肖 佳 ，邢飞跃 ，George L. Tipoe (1. 暨南大学生命科学技术学院免疫生物学系, 广东广州 510632 ；2. 香港大学李嘉诚医学院解剖 学系，中国香港) 摘要：环氧合酶-1 （COX-1 ）是COX 家族中组成性表达的成员。为探讨 COX-1 在急性肝损伤中的作用和机制， 通过注射四氯化碳（CCl4 ）诱导野生型或COX-1 敲除小鼠的急性肝损伤，研究各种损伤表征的变化。结果表明， 完全或部分敲除 COX-1 基因、使用药物抑制 COX-1 酶活性均可恶化四氯化碳造成的急性肝损伤，主要表现在： （1）恶化的肝脏组织学和血清中转氨酶的变化；（2 ）通过CYP2E1 介导的氧化压力的恶化；（3 ）升高的肝脏 炎症反应；（4 ）恶化的肝脏凋亡反应。研究表明，肝脏中COX-1 的基本表达水平对于肝脏抵御化学急性损伤非 常重要。 关键词：细胞生物学；环氧合酶；四氯化碳；前列腺素；急性肝损伤 Cyclooxygenase-1 Serves a Vital Hepato-Protective Function in Chemically Induced Acute Liver Injury XIAO Jia 1,2, XING Feiyue 1, George L. Tipoe 2 (1. Department of Immunobiology, Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China; 2. Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China) Abstract: Cyclooxygenase-1(COX-1) is the constitutive form of the COX enzyme family which produces bioactive lipids called prostanoids. Although the role of COX-2 in liver diseases has been studied, little is known about the function of COX-1 in liver injury. We aimed to find out the role and mechanism of COX-1 in acute liver injury. Carbon tetrachloride (CCl4) was administered in to induce acute liver injury in wild-type or COX-1 deficient mice. Both genetic (partially or completely) deletion of COX-1 expression and pharmacological inhibition of COX-1 activity in mice exacerbated acute liver in