小鼠卵巢组织的超速冻存法研究_庞龙.pdfVIP

2002 9 Sep., 2002 10 　3 ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA Vol.10　No.3 庞龙　王燕蓉　沈新生　张崇智 (, 　750004) 　　【】　　, , , 。 　 , 、、。 、、、。 　 , ; ;、, 。 【】　, ;; +2 【】Q813.1 　　【】A　　【】1005-4847(2002)03-0142-06 The Research of Overspeed Method to Cryopreserve Mice Ovarian Tissue PANG Lo g, WANG Ya ro g, SHEN Xi she g , ZHANG Cho gzhi (Histology a d Embryology Departme t of Ni gxia Medical College, Yi chua 　750004, Chi a) 【Abstract】　Objective　To study the overspeed method of cryopreservatio of mice ovaria tissue, a d fi d out a co ve- ie t a d practical method of its storage.Methods　After thawi g, the ovaria tissueswere tra spla ted.The recovered time a d rate of estrus, level of serum estradiol of recipie t mice, e zyme histochemistry of tra spla tatio tissues, the ultrastructure of thawed tissues were studied to compare the cryopretective effect of cryopreservatio methods a d types of CPA.Results and con- clusion　I compariso with procedure freezi g method, though the same CPA, the liquid itroge vapour freezi g method showed the adva tage of well ultrastructure of ovaria tissue i additio to the same results of e zyme histochemistry.There were o sig ifi- ca t differe ce betwee the procedure freezi g method a d liquid itroge vapour freezi g method i recovered rate of estrus, level of serum estradiol. 【Key words】　Mice, ovary;Liquid itroge vapour freezi g method;Procedure freezi g method 　　 。 [2] , 。 , 20 50 。90 。 , Carroll [1] , , , [] (C206);