高效液相色谱法测定蔬菜中精喹禾灵残留的研究_马建明.pdfVIP

2007 5 17 5 Chi ese Jour al ofH ea lth Laboratory Tech ology, M ay 2007; Vol 17 No 5 827 高效液相色谱法测定蔬菜中精喹禾灵残留研究 马建明, 赵立达, 马旭亮 ( , 315300) [ ] : : // , , XDB C ( 150 mm @ 416 mm i1 d1, 5Lm ), / 8 - ( 10 mmol/L, pH 61 2, 80/20, v/ v) , 01 8m l/m i , 35e , 235 m: 8312% ~ 9412% , R SD 51 5% , 01 5~ 501 0 m g/L ( r = 01 9992): , [ ] 高效液相色谱; 蔬菜; 精喹禾灵 + [ ] O65717 2 [] A [] 1004- 8685(2007) 05- 0827- 02 D eterm ination of qu izalofop- p- ethyl residue in vegetab les by HPLC M a J ian-m ing, Gong W en-j ie, M a X u-liang ( C ixi C e ter for Disease Co trol a d Preve tio , C ixi 315300, Ch i a) [ Abstract] O b ective: To establish a smi p le, accurate a d se sitive m ethod for the determ i atio of qu izalofop- p- ethyl i vegetab les by liqu id chrom atography w ith ultraviolet detectio 1M ethods: Sam plesw ere extracted by metha ol/aceto e/210 m ol/L hydroch loric acid a d clea ed up o a eutral alum i a a d florisilm ixed colum 1A satisfactory separatio w as achieved o a zorb- ax XDB C colum ( 150 mm @ 41 6 mm i1d1, 5Lm ) usi gm etha ol/ acetic acid- amm o ium acetate ( 10 mmol/L, pH 612, 80/ 8 20, v /v) as them ob ile phase at a co sta t temperature of 35e at a flow rate of 01 8m l/m i 1Detectio was perform ed o a ultra- violet detector at 235 m 1Resu lts: The li ear ra gew as 01 5~ 5010 mg/L a d the lmi it of qua tificatio w as fou d to be 01 5 m g/ L1The extractio recoveriesw ere betwee 831 2% ~ 9412% , theR SD w as less tha 515% 1 Conc lu sion: Th is described method is fou d to be smi ple, rap id a d accurate for