荣骨定痛膏对骨性关节炎模型大鼠炎性细胞和细胞因子的影响.pdfVIP

·论 著 · 荣骨定痛膏对骨性关节炎模型大鼠炎性细胞及细胞因子的影响  刘晓霞 1 1* 2 2 1 1 ，王志旺 ，刘彩民 ，陈小平 ，妥海燕 ，王瑞琼 (1.甘肃中医药大学，兰州 730000 ；2.靖远县中医院，甘肃 靖远 730600) 摘要：目的 研究荣骨定痛膏对骨性关节炎模型大鼠炎性细胞及细胞因子的影响。方法 采用注射 4%木瓜蛋白酶复制骨 性关节炎大鼠模型，观测膝关节功能、关节滑膜厚度和关节滑液中炎性细胞数，酶联免疫法检测血清 IL-1β、IL-6 、TNF-α。 结果 荣骨定痛膏可明显减小骨性关节炎大鼠关节滑膜肿胀厚度，扩大膝关节的活动范围，减少关节滑液中炎性细胞数， 降低血清 IL-1β、IL-6 与 TNF-α的水平。结论 荣骨定痛膏对骨性关节炎具有一定的治疗作用，缓解关节炎症、降低 IL-1β、 IL-6 、TNF-α水平可能是其作用机制之一。 关键词：荣骨定痛膏；骨性关节炎；IL-1β；IL-6 ；TNF-α 中图分类号：R285.5 文献标志码：A 文章编号：1007-7693(2015)07-0777-04 DOI: 10.13748/ki.issn1007-7693.2015.07.001 Effects of Ronggu Dingtong Ointments on Inflammatory Cells and Cytokines in Rats with Osteoarthritis 1 1* 2 2 1 1 LIU Xiaoxia , WANG Zhiwang , LIU Caimin , CHEN Xiaoping , TUO Haiyan , WANG Ruiqiong (1.Gansu University of Chinese Medicine, Lanzhou 730000, China; 2.Jingyuan Hospital of Traditional Chinese Medicine, Jingyuan 730600, China) ABSTRACT: OBJECTIVE To explore the effects of Ronggu Dingtong ointments(RGDT ointments) on inflammatory cells and cytokines in rats with osteoarthritis (OA). METHODS OA model was established by injecting 4% papain in articular cavity. The function of knees, synovial thickness, and the content of inflammatory cells in synovial fluid were observed. ELISA kits were used to measure the levels of IL-1β, IL-6 and TNF-α. RESULTS RGDT ointments increased the movable degree of knees, decreased the synovial swelling thickness of joints and the levels of IL-1β, IL-6 and TNF-α in serum. CONCLUSION RGDT ointments has some therapeutical effects on OA, and one of the mechanisms may be remitting inflammation of joint and decreasing the levels of IL-1β, IL-6