生物工程下游技术-第三章.pdf

Chapter Three Microcarrier Specifically for Cell Culture 第三章 细胞培养专用微载体 Many cell lines are said to be “anchorage dependent,” which means that in order to grow and divide they must attach themselves to a suitable surface. At the research scale they are typically grown in small plastic flasks or plates. However, large-scale production of anchorage- dependent cells or cell derived products has had to rely on methods other than a simple linear expansion of this process. Traditionally, roller bottles have been employed for this task. The rolling action ensures that the cells are alternately exposed to growth medium and oxygen. The roller bottle method however, is very cumbersome and expensive for the production of large quantities of cells for a number of reasons: the roller bottles are an inefficient use of space, they require extensive handling, labor and medium, and the process is difficult to monitor. Over the years a number of schemes for growing large quantities of anchorage- dependent cells have been examined. These include spiral film, plastic bags, packed or fluidized beds, hollow fiber beds, and microcarrier suspension culture. In our experience, microcarrier suspension culture has been the most successful of the different approaches. Microcarrier Suspension Culture The microcarrier technique was first developed at