红曲菌株RAPD多态性分析.pdfVIP

2007 9 31 5 RAPD 310053 : [ 目的] 研究红曲分离株的遗传多样性和亲缘关系, 为种质鉴定保护和利用提供参 [ 方法] 用随机引物对10 个红曲菌 株进行RAPD 分析, 从中筛选出63 特征性好, 多态性高的RAPD 图谱, 用NT SYSP C21 软件进行同一性和差异性分析并根据 遗传距离构建系统进化树[ 结果] 红曲分离株间的同一性平均7332% , 表明它们的遗传背景具有很大的相似性; RAPD 聚类 结果与菌株的形态差异相关, 表明RAPD 分子标记进行菌株鉴别与传统形态分类鉴别结果基本一致, 鉴别能力较强[ 结论] 基 于RAP D 多态性的遗传分析能从分子水平上揭示红曲菌的遗传背景差异, 为分类鉴定种质起源和系统进化提供辅助手段和技 术依据 : 红曲菌; RAP D 分析; 遗传同一性; 系统进化树 : R284 : A : 2007) Random AmplifiedPolymorphicDNAAnalysis ofMonascus Strains Din H on m ei, Ge Ernin , Wan Zeshi Zhej iang ChineseM edi cal University, H angz hou( 310053) , China Abstract: [ Object ive] T o st udy the enet ic diversity and relative relationship of different Monascus trains to provide reference for classification and protection of erm plasm resources. [ Methods] Comparin the results of the random amplified polymorphic DNA ( RAPD) fin erprints derived from 10 cult ivated strains of Monascus, 63 RAPD electrophoresis atlases were selected to calculat e the enetic identity and diver ence amon them by NTSYSPC21 soft ware. And then phylo enetic t ree of Monascus strains w as conceived accordin to their enetic distance by clusterin method. [ Results] It w as su ested t hat the comparability of enet ic back rounds was plenty because of 7332% avera e identity of Monascus strains. The results of RAPD analysis w ere consistent w ith that of morpholo ic st udy, so the RAPD molecular m arker technolo y is an effective way to identify Monascus strains. [ Con clusion] RAPD analysis can reveal t he differentia of enetic back rounds of M onascus st rains, and it could be applied w idely in clas sification and identification, erm plasm ori in and evolution. Key ords: M onascus; RAPD analysis; enetic ident ity; phylo enetic tree 11 , 111 : 1~ [ 14]