鲟源致病性嗜水气单胞菌X的分离.doc

鲟源致病性嗜水气单胞菌X1的分离鉴定与药敏特性研究 李圆圆* 曹海鹏* 何 珊 杨先乐** (农业部渔业动植物病原库 上海海洋大学 上海 200090) 摘 要: 从患细菌性败血症的西伯利亚鲟(Acipenser baerii)的体内分离到一株致病菌株X1, 其对西伯利亚鲟的半数致死浓度(LC50)为5.62×105 CFU/mL, 具有较强毒力; 菌株X1为革兰氏阴性杆菌, 菌体大小为1.0 μm~1.2 μm×2.1 μm~2.4 μm, 周生侧鞭毛, 在兔血琼脂平板上能形成明显的β-溶血圈, 经ATB细菌鉴定仪鉴定和16S rDNA序列分析, 菌株X1为嗜水气单胞菌(Aeromonas hydrophila)(登录号：EU669667); 其系统发育分析表明, 菌株X1与嗜水气单胞菌ATCC35654(登录号：X74676.1)的亲缘关系最近, 其同源性为99％。此外, 菌株X1对先锋必、左氟沙星等2种药物高度敏感, 对妥布霉素、氟哌酸、舒普深、卡那霉素、庆大霉素、复达欣、万古霉素、新霉素、多粘菌素B、洛美沙星等10种药物中度敏感。 关键词: 西伯利亚鲟, 嗜水气单胞菌, 鉴定, 药敏特性 Isolation and Identification of Aeromonas hydrophila Strain X1 from Acipenser baerii and Its Antibiotic Sensitivity LI Yuan-Yuan* CAO Hai-Peng* HE Shan YANG Xian-Le** (Aquatic Pathogen Collection Center of Ministry of Agriculture, SHOU, Shanghai 200090) Abstract: A pathogenic bacterial strain X1 was isolated from Siberian sturgeon (Acipenser baerii) suffering with bacterial septicemia. The 50% lethal concentration (LC50) of strain X1 was 5.62×105 CFU/mL, which showed that strain X1 was rather strong virulent to Acipenser baerii. Strain X1 was gram negative and 1.0 μm~1.2 μm × 2.1 μm~2.4 μm in size with peritrichous flagella, and had β-hemolytic activity on rabbit blood agar. By means of ATB expression identification and 16S rDNA sequence analysis, strain X1 was identified as Aeromonas hydrophila (locus number: EU669667), which was the closest relative to Aeromonas hydrophila strain ATCC35654 (locus number: X74676.1) with 99% homology. In addition, strain X1 was highly sensitive to cefoperazone and cravit, and intermediately sensitive to ten kinds of antibiotics including tobramycin, norfloxacin, sulperazone, kanamycin, gentamycin, fortum, vancomycin, neomycina, polymyxin B and lomefloxacin. Keywords: Acipenser baerii, Aeromonas hydrophila, Identification, Antibiotic sensitivity西伯利亚鲟(Acipenser baerii)隶属于鲟科(Acipenserdae)鲟属(Acipenser), 是现存26种鲟类中的一种, 主要分布于俄罗斯西部的颚毕河至东部的科雷马河之间的西伯利亚各河流之中[1]。西伯利亚鲟具有生长速度快、适应性强、肉质好、鱼籽酱品质高等优点, 已推广至德国、法国和匈牙利等国进行人工养殖[2]。西伯利亚鲟自1996年首次被中国