噬菌体裂解酶_抗菌作用与药物开发研究.pdfVIP

Chinese Journal of New Drugs 2009, 18( 10) # # : 1 2 2 2 , , , ( 1浙江树人大学生物与环境工程学院, 杭州 310015; 2浙江工业大学药学院, 杭州 310032) [] 近几十年来, 抗生素的滥用导致致病 耐药性问题日益严重, 已经对人类健康造成极大威胁 噬 体裂解酶作为 一种新的治疗手段开始受到人们关注裂解酶是噬 体在感染宿主 末期表达的蛋白 质, 它通过水解细 细胞壁的肽聚糖使子代噬 体释放已有实验证明裂解酶在体外能高效且特异性地杀 死细 文中结合作者的研究工作, 综述了噬 体裂解酶的抗 机制结构特征抗 作用以及利用基因工 程手段制备重组噬 体裂解酶等方面的研究进展, 并探讨了噬 体裂解酶作为新型抗 药物的研究开发重 点与应用前景 [] 噬 体裂解酶; 抗 机制; 抗 作用 [] R915; Q939. 48 [] A [] 1003- 3734( 2009) 10- 0891- 04 Bacteriophage lysin s: antibacterial effects and m edical app lication 1 2 2 2 CHEN W ei-qing, WANG X iao-feng, WANG Pu , WU M in ( 1College of B iolog ical and EnvironmentalEngineering, Zhej iang Shuren University, H angzhou, 310015, China; 2 College of Pharmaceutical Science, Zhej iang University of echnology, H angzhou 310032, China) [ Abstract] In recent decades, drug-resistance of pathogen ic bacteria resulting from antib iotics abuse has greatly threatened human health. Bacteriophage lysins as new therapeutics have begun to attract peopleps attention. Bacteriophage lysins are cellw all hydrolysing enzymes synthesized during late gene expression in the lytic cycle of phages mu ltiplication; thereby they hydrolyze the peptidoglycan and enable the release of progeny phages. Studies have indicated that lysins can k ill bacteria efficiently and specifically in vivo. In th is article, new progress in the research ofm echan ism, structural characteristics, antibacterial activity of bacteriophage lysins, asw ell as recom- b inant lysins preparation by genetic engineering, w ere review ed in comb ination w ith authorps