第卵巢切除大鼠脑内雌激素受体α表达的变化.docVIP

第卵巢切除大鼠脑内雌激素受体α表达的变化 　　作者：金丽英 郭宗君 崔红波 兰克涛 杜芳 (1 青岛大学医学院脑血管病研究所，山东 青岛 266003; 2 兖州矿务局医院神经内科; 3 青岛市第三人民医院急诊内科) 　　[摘要] 目的 观察卵巢切除大鼠脑内不同部位雌激素受体Α(ERΑ)表达变化。方法 根用Singh等方法制作大鼠卵巢切除模型(模型组)，免疫组织化学染色观察海马CA1区、皮质区、杏仁复合体区、基底前脑Meynert核区等部位ERΑ阳性细胞表达变化，并与手术对照组(对照组，不切除卵巢)比较。结果 对照组大脑各观察区有大量的ERΑ表达。与对照组比较，模型组大脑海马CA1区、大脑皮质区、杏仁复合体区、基底前脑Meynert核区ERΑ阳性细胞数显著减少(t=2.68～5.68,P0.01)。结论 卵巢切除可致大鼠脑内多部位ERΑ表达减少，这种病理变化可能与轻度认知障碍的发生有关。 　　[关键词] 受体，雌激素;卵巢切除术;认知障碍;大鼠，Wistar 　　OBSERVATION OF ESTROGEN RECEPTOR Α IN DIFFERENT CEREBRAL REGIONS IN OVARIECTOMIZED RATSJIN LIYING， GUO ZONGJUN， CUI HONGBO, et al (Institute of Cerebrovascular Diseases, Qingdao University Medical College, Qingdao 266003, China) 　　[ABSTRACT]ObjectiveTo observe the changes of estrogen receptor Α (ERΑ) in different cerebral regions in ovariectomized rats. MethodsAdult healthy female Wistar rats were randomly divided into two groups: model group(OVX group) and control group. The expression of ERΑ masculine cells in the hippocammpi CA1, cortex, amygdala, Meynert of the basal forebrain were compared between the two groups.ResultsThe expression of ERΑ masculine cells was observed in every section of the brain in control group, while in model group，and the ERΑ masculine cells in the cortex and Meynert nucleus were distinctly decreased compared with the control group (t=2.68-5.68, P0.01). There was no difference in the hippocampi CA1 and amygdala between the two groups.ConclusionThe expression of ERΑ masculine cells was decreased in the cortex and Meynert nucleus of the brain of ovariectomized rats. The change may be related to the neurodegeneration of acetylcholine nerve cells from Meynert nucleus to the cortex. 　　[KEY WORDS]receptors, estrogen; ovariectomy; cognition disorders; rats, Wistar 　　雌激素由芳香化酶催化雄激素转化而来，影响其靶组织的生长、分化和功能发挥。雌激素对神经系统的功能具有一定的保护作用[1]。研究表明，女性体内的雌激素缺乏与轻度认知障碍(MCI)发病有关[2]。大鼠卵巢切除(OVX)是一种较好的雌激素缺乏模型[3]，应用这种模型观察大鼠脑内不同部位的雌激素受体Α(ERΑ)表达的变化及病理改变，可以探讨雌激素缺乏是否影响ERΑ的改变，从而了解其与MCI发生有关的病理机制。 　　1 材料和方法 　　1.1 实验动