中国奠基群奥利亚罗非鱼遗传多态性DNA指纹图谱3.pdfVIP

南京农业大学学报 　2000 , 23 (3) : 61～63 　 Journal of Nanjing Agricultural University 中国奠基群奥利亚罗非鱼遗传多态性的DNA 指纹图谱 王进科 　夏德全 　吴婷婷 (南京农业大学动物医学院 , 南京 210095) 摘要 　运用 336 和 3315 两种人源小卫星探针对中国奠基群奥利亚罗非鱼群体进行遗传多态性的DNA 指纹图谱研究。结 果表明 , 在奥利亚罗非鱼群体中两种探针都能产生个体特异性的 DNA 指纹图谱 ; 两种探针平均检测出 625 条条带 , 其 中多态性条带占585 % , 个体平均条带数为 7813 ; 奥利亚罗非鱼种群内相似系数为 0740 , 遗传变异系数为 0260 , 证 明奥利亚罗非鱼群体的遗传纯度极高 , 遗传多态性很低 , 但仍存在一定的遗传变异 ; DNA 指纹图谱中 336 产生的 72 kb 条带和 3315 产生的 94 kb 条带为奥利亚罗非鱼群体内各个体所共有 , 并且表现出一致的单态性和较强的杂交信号 , 反 映了奥利亚罗非鱼在该位点小卫星的群体特征 , 可作为奥利亚罗非鱼特有的分子遗传标记。 关键词 　奥利亚罗非鱼 ; 遗传多态性 ; DNA 指纹图谱 分类号 　S965125 Studies on genetic polymorphism of O . aureus of founder in China with DNA fingerprinting Wang Jinke , Xia Dequan and Wu Tingting (College of Veterinary Medicine , Nanjing Agric Univ , Nanjing 210095) ABSTRACT 　The genetic polymorphism of O. aureus of founder in China was studied with nonradioactive DNA fingerprinting with human minisatellite probes 336 and 3315. The results showed that both probes could be used to produce individual distinctive DNA fingerprints on O. aureus . Each probe could detect out 625 bands on studied fishes , among which 585 % was polymorphic , and average individual band was 7813. The withinpopulation similarity index of O. aureus was 0740 and variation coefficient was 0260 , evidently , the ge netic purity of O. aureus population was very high and genetic polymorphism was low , however , there was certain variation in O. au reus . The band of 72 kb produced by probe 336 and 94 kb produced by 3315 in DNA fingerprints were shared by each individual and showed co