Celecoxib通过抑制Survivin表达促进肺癌A549细胞凋亡.docVIP

Celecoxib通过抑制Survivin表达促进肺癌A549细胞凋亡 作者:龙伟,周锐 作者单位：1.湖南省吉首大学医学院临床医学系，湖南 吉首 416000；2.中南大学湘雅二医院，湖南 长沙410011 【摘要】 　　［目的］研究 Celecoxib对肺癌A549细胞生长和凋亡的影响并探讨其作用机制。［方法］用Western blot法检测Celecoxib抑制肺癌A549细胞后生存素（Survivin）表达的变化；用酶联免疫吸附法（ELISA）测定培养上清液中前列腺素2（prostaglandin-2，PGE2）含量的变化；用四唑盐（MTT）比色法检测细胞增殖；流式细胞仪测定细胞周期。［结果］ 随着Celecoxib剂量的增加和时间的延长，PGE2的含量减少，survivin表达也相应减少，细胞增殖减少而凋亡增加。［结论］Celecoxib可以通过PGE2的途径下调A549细胞survivin的表达并促进细胞凋亡。 【关键词】 A549细胞　Celecoxib　前列腺素2　环氧合酶2　生存素 　　Celecoxib Promote Apoptosis in Human Lung Cancer A549 Cells by Inhibiting Survivin LONG Wei1, ZHOU Rui2 (1.Medicine Institute of Jishou University, Jishou 416000, China; 2.Xiangya Second Hopital of Central South University, Changsha 410011, China) Abstract： ［Purpose］ To investigate the effect and mechanism of Celecoxib in inducing proliferation inhibition and apoptosis in human lung cancer A549 cells.［Methods］ The expression of survivin through Celecoxib blockading were detected by Western Blot. ELISA were used to detect the change of prostaglandin-2 (PGE2) content in the supernatant of culture solution. The anti-proliferative effect was measured by using Methabenzthiazuron (MTT) assay. Cell cycle and apoptosis were analyzed by using flow cytometry. ［Results］ With the blockade of Celecoxib increased, the PGE2 content reduced and the expression of survivin weakened, but the apoptosis rised. ［Conclusion］ Celecoxib can down-regulate the expression of survivin and then promote the apoptosis of A549 cells via PGE2 pathway. Key words: A549 cell; Celecoxib; PGE2; COX-2; survivin Celecoxib是COX-2的特异性抑制剂，最初用于治疗类风湿性关节炎和骨关节炎，近十几年来发现其具有抗肿瘤作用，作用机制可能与抑制肿瘤细胞增殖并诱导其凋亡，且抑制肿瘤血管生成并对周围组织的粘附侵袭有关。Survivin是近年来发现的抗凋亡蛋白（inhibitor of apoptosis protein，IAP）家族的新成员，在人类绝大多数恶性肿瘤中均有表达，通过对抗细胞凋亡而有利于肿瘤的生长。目前，有关Celecoxib与survivin的关系报道极少。我们研究的目的是要观察Celecoxib对肺癌A549细胞survivin表达的影响，探讨Celecoxib调节survivin表达进而促进A549细胞凋亡的分子机制。 1 材料与方法 1.1 材 料 人肺癌A549细胞来自于中