番石榴叶提取物对高脂性肥胖小鼠的减肥降脂作用的研究（.doc

番石榴叶提取物对肥胖小鼠的减肥降脂作用的研究( 李璇1,2 蔡国平1,2△ （1 清华大学生命科学院 北京100084；2 清华大学深圳研究生院生命学部 广东深圳 518055深圳清华大学研究院深圳市创新中药及天然药物研究重点实验室 广东深圳518057） 摘要：目的：研究番石榴叶提取物对小鼠肥胖模型的减肥、降脂作用。方法：①利用高脂饲料喂养，建立小鼠肥胖模型；②小鼠肥胖模型初步建立后，以体重、热量摄取、腹部脂肪系数、FBG、TG、TC、HDL-C、LDL-C为指标，通过与非诺贝特的比较，番石榴叶提取物的减肥、降脂及其剂量关系。结果：①与比较，组的小鼠体重、热量摄取、腹部脂肪系数、FBG、TG、TC、LDL-C显著增加；②给药后，3个剂量番石榴叶提取物组的小鼠体重、腹部脂肪系数、FBG、TG、TC、LDL-C均明显低于高脂饲料组，且呈显著或极显著差异，并与阳性对照组无明显差异；③3个剂量番石榴叶提取物组的减肥、降脂作用存在一定的效应-剂量关系，即组（50 mg/Kg）组（100 mg/Kg）组（200 mg/Kg）。结论：番石榴叶60 %乙醇提物对小鼠肥胖症和高脂血症有一定的预防和治疗作用，3个剂量组中组（200 mg/Kg）效果最好，可望开发为减肥降脂的纯天然药物。 关键词：番石榴叶；肥胖；高脂血症中图分类号： R285.5 文献标识码：A 文章编号： Effect of Extracts from Psidium guajava Leaves on Anti-obesity and Lipid Lowering Activity in High-fat Diet Induced bese Mice LI Xuan1,2, LI Xiao-fan3, CAI Guo-ping1,2△ (1 School of Life Science, Tsinghua University, Beijing 100084, China;2 Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen Guangdong 518055, China;3 Key Lab for New Drugs Research of Traditional Chinese Medicine in Shenzhen, Research Institute of Tsinghua University in Shenzhen, Shenzhen Guangdong 518057, China) ABSTRACT: Objective: To investigate the effect of extracts from Psidium guajava leaves on anti-obesity and lipid lowering activity in high-fat diet induced mice. Methods: ①A model of obesity mice was established by high-fat diet feeding. ②Compared with fenofibrate, weight, visceral fat accumulation, FBG, TC, TG, HDL-C and LDL-C were measured as indicators to verify the effect of extracts from Psidium guajava leaves on lipid lowering activity in high-fat diet induced mice, moreover their dose-response effects were determined respectively. Results: ①In contrast to the Nomal Group, the HFD Group had a significant increase in weight, visceral fat accumulation, FBG, TC, TG and LDL-C. ②After administration of extracts from Psidium guajava leaves, the relevant indicators in three groups treated with different doses were significantly down-regulated, compared with HFD, while no significant d