_硫辛酸对AGEs作用下血管内皮细胞PD_L1表达的影响.pdfVIP

20 11 2 37 3 J iang su M ed J, Feb ruary 20 11, V ol 37, N o . 3 249 A GEs PDL 1 杜 宣 施毕旻 陈永井 张学光 ( L A) ( A G Es) ( H U V EC) 1( PDL 1) P DL 1 ( sPDL 1) : ( C : R PM I 1640 ) ; ( A GEBSA ) [ AG EBSA 50 mg/ ml( A G50 ) 200 mg/ ml( A G200 ) 400 mg/ ml( A G400 ) ; ( BSA ) [ BSA 50 mg/ ml( BG50 ) 200 mg/ ml ( BG200 ) 400 mg/ ml ( BG 400 ) ; [ R PM I 1640 + 200 mo l/ L L A ( C + L A 200 ) 200 mg/ ml AG EBSA + 200 mo l/ L L A ( A G200 + L A 200 ) 72 h , H U V E C PDL 1 sPDL 1 A G EBSA 72 h , P DL 1 sPDL 1 ( P 00 1) , L A , A G200 + LA 200 PDL 1 sPDL 1 AG 200 ( P 005) A G Es PDL 1 , sPDL 1 ; L A AG Es ; ; 1 R587 A 2011) Eff ect of alphalipoic acid on the expression of programmed death ligands1 in human umbilical vein endothelial cells under advanced glycosylation end products DU Xua n , SHI Bimin , CHEN Y ongj ing , et al . D epar tment o f Endocrinology , Aff ilia ted First Ho spita l , So ochow University , Suzho u 215006, CHIN A Abstract Obj ective T o inv est igate the eff ect of alphalipo ic acid ( LA ) on the expressio n of pr og rammed death ligands1( PDL 1) o f human umbilical vein endothelial cells ( H U V ECs) and the level of soluble P DL 1 ( sP DL 1) in the super natant treated by advanced g ly co sylation end product s ( A GEs) . Methods HU VE Cs w ere rando mly div ided into four g ro up s o f co ntr ol ( C, incubated w it h RPM I 1640 alo ne ) , AG Ebo vine ser um albumin ( AG EBSA ) ( A G50 , AG 200 , A G400 , incubated w it h AG EBSA 50 , 200 and 400 mg/ ml, respect ively) , BSA ( BG50 , BG200 and BG 400 , incubated w ith BSA 50 , 200 and 400 mg/ ml, respectively) , L A ( C + L A 200 , incubated w ith RP M I 1640 plu s L A 200 mol/ L , AG 200 + L A200