1型糖尿病小鼠模型的构建.pdfVIP

国际检验医学杂志2011年9 月第32 卷第14期 Int J Lab Med, September 2011, Vol.32,No.14 # 1589 # # # 1 1, 2 1v 谭 潇 ,肖建华 ( 1. 南华大学病原生物学研究所, 湖南衡阳421001;2.邵阳医学高等专科学校, 湖南邵阳422000) : 构建 1 型糖尿病小鼠 型, 为进一步研究1 型糖尿病发病机制及治疗方法提供依据 型的诱导采用 多次小剂量链脲佐菌素(STZ) 给药法( MLDST Z) , 雄性B LB/ c 小鼠64 只, 随机分为3 组: 型组阴性对照组正常对照组 型组小鼠连续5 d 腹腔注射ST Z 溶液, 阴性对照组腹腔注射柠檬酸缓冲液,正常对照组不注射给药后观察小鼠一般状况, 检测 血糖尿糖, 同时以石蜡切片观察小鼠的胰腺组织病理变化 STZ 诱导的糖尿病 型鼠与对照相比存在明显的多饮多 食多尿和体质量减轻等典型的糖尿病表现在末次注射STZ 3周后, 型组小鼠尿糖均为阳性, 对照组均为阴性在4 周后, 型组小鼠空腹血糖水平明显高于对照组, 差异有统计学意义( P 0. 01) 型鼠均有不同程度的胰岛萎缩胰岛B 细胞空泡变 性数目减少及不同程度的胰岛炎改变 采用 MLDSTZ 构建的1 型糖尿病小鼠 型稳定, 方法可靠 :糖尿病; 小鼠; 型, 动物; 链脲佐菌素 DOI: 10.3969/j.issn. 1673-4130.2011. 14.029 : : 1673-4130(2011) 14-1589-03 Construction of mouse models of type 1 diabetes mellitus 1, 2 1v TanXiao ,XiaoJianhua (1.N anhua University Pathogenic Biology Research Institute,H engyang H unan 421001, China; 2. Shaoyang edical College, Shaoy ang H unan 422000, China) Abstract:Objective To construct mouse models of type 1 diabetes mellitus(T 1DM) to provide the evidences for researches on the pathogenesis and therapeutic method.Methods Mouse models of T 1DM were induced through enterocoelia limosis injection of streptozotocin ( MLDST Z) . 64 male B LB/c mice were randomly divided into three groups, including model group, negative con- trols and normal controls.Model mice received intraperitoneal injection continuously for 5 days with STZ solution,negative controls received intraperitoneal injection with citric acid buffer, and normal controls group were not treated. T