2型糖尿病初发患者外周血单核细胞中核因子_kB表达及超敏C反应蛋白水平研究.docVIP

2型糖尿病初发患者外周血单核细胞中核因子_kB 表达及超敏C反应蛋白水平研究 　　作者：楼大钧，朱麒钱，斯徐伟 作者单位：绍兴市人民医院内分泌科，浙江绍兴 　　【摘要】 目的 探讨2型糖尿病(T2DM)初发患者外周血单核细胞中核因子kB(NF_kB)的表达及血清超敏C反应蛋白水平(hs-CRP)。方法 颗粒增强免疫沉淀法测定91例初发T2DM患者(G2组)及60例对照组(G1组(血清超敏C反应蛋白水平(hs_CRP)水平;免疫组织化学染色法测定G2、G1组外周血单核细胞NF_kB活性;分析NF_kB活性与hs_CRP浓度相关性。结果 G2组外周血单核细胞中NF_kB活性和血清hs_CRP水平显著高于G1组(P0.01)，而且外周血单核细胞中NF_kB活性与血清hs_CRP水平显著正相关。结论 2型糖尿病患者处于炎症状态，血单核细胞中核因子NF_kB表达及血清hs_CRP水平升高可能与动脉粥样硬化的形成有关。 　　【关键词】 2型糖尿病,核因子_kB,超敏C反应蛋白 　　Changes of Nuclear Factor_ΚB Activity in Peripheral Mononuclear Cell and Serum High_Sensitivity C Reactive Protein in Newly Diagnosed Type 2 Diabetes (T2DM). LOU Da_jun, ZHU Qi_qian, SI Xu_wei. Department of Endocrinology, Shaoxing Peoples Hospital, Zhejiang 312000, China 　　[Abstract] Objective To investigate the changes of Nuclear Factor_ΚB (NF_ΚB) in peripheral mononuclear cell and serum High_sensitivity C reactive protein (hs_CRP) in T2DM. Methods Immunohistochemical method was used to measure activity of NF_ΚB in peripheral blood mononuclear cell and immunolurbidimelric assay was used to measure hs_CRP in 91 T2DM and 60 normal control subjects (control group). At the same time linear correlation analysis was used to analyze the correlation between the level of hs_CRP and the level of NF_ΚB activity. Results The expression level of NF_ΚB in peripheral mononuclear cell and serum hs_CRP concentration in T2DM were significantly higher than those in control group(P0.01). The serum hs_CRP level was positively correlated with NF_ΚB activity in peripheral blood mononuclear cells. Conclusions Type 2 diabetes had higher inflammation factors level.The increased activity of NF_KB and hs_CRP in T2DM may contribute to atherogenesis. 　　[Key words] Type 2 diabetes; Nuclear factor kappa_B; High_sensitivity C reactive protein 　　近年来认为低度炎症过程与肥胖、2型糖尿病(T2DM)、动脉粥样硬化的发生发展密切相关。严格控制血糖可以明显降低糖尿病患者微血管病变的发生率，但并没有显著降低大血管病变的发生率，其原因之一认为是炎症反应，外周血单核细胞的激活侵入动脉内膜形成泡沫细胞，在动脉粥样硬化形成早期起重要作用[1]。研究发现NF_kB是炎症的核心调节因子，动脉粥样硬化炎证反应过程所涉及的许多炎症因子都是由NF_kB激活的[