NGF和GM1联合应用对坐骨神经损伤大鼠初级传入神经元的保护作用.pdfVIP

44 4 ( ) Vol. 44 No. 4 2006 4 JOURNAL OF SHANDONG UNIVERSITY(HEALTH SCIENCES) Apr. 2006 : 1671- 7554(2006) 04- 0332- 04 NGF GM1 1,2 1 1 1 1 1 1 , , , , , , ( 1. , 250012; 2. , 256603) [ ] 目的: 探讨联合应用神经营养因子(NGF) 和神经节苷脂( GM1) 对大鼠周围神经损伤后初级 传入神经元的保 作用方法: 选用SD 大鼠96 只, 随机分为对照组NGF 组GM1 组和NGF + GM1组 将大鼠右侧坐骨神经切断, 神经两断端相距5 mm, 硅胶管桥接, 用不同的药物处理后, 于术后不同时间取 出背根神经节进行研究结果: 4 周时神经元数和神经传导速度, NGF+ GM1 组多于或快于其它各组 (P 0. 01) , NGF 组和GM1 组均多于或快于对照组(P 0. 01) ; 8 周时, 各实验组多于或快于对照组( P 0. 01) , 各实验组之间差异无统计学意义(P 0. 05) 结论: 周围神经损伤后, 联合应用NGF 和GM1 对初 级传入神经元的保 作用优于单用NGFGM1, 且起效早 [ ] 神经生长因子类; 神经节苷脂; 神经元; 神经节; 感觉 [ ] R322. 8; R332 [ ] A Protective effects of NGF an GM1 on primary sensory neurons in SD rat with sciatic nerve injury 1, 2 1 1 1 1 1 1 HUANG Fei , WANG Huaijing , XING Yi , GAO Wei , LI Yonggang , XING Ziying , LI Zhenzhong ( 1. Department of Anatomy, School of Me icine Shan ong University, Jinan 250012, Shan ong, China; 2. Binzhou Me ical College, Binzhou 256603, Shan ong, China) [ABSTRACT] Objective: To observe the protective effects of nerve growth factor ( NGF) an monosiagan gliosi e ( GM1) on primary sensory neurons in ratswith sciatic nerve injury. Methods: Ninetysix SD ratswere ran omly ivi e into normal saline control group(A) , NGF group( B) , GM1 group( C) an NGF + GM1 group( D) . The sciatic nerve was incise with a 5 mm efect. The two en s of nerve were connecte with a 10. 0mm length sili cagel tube of 2. 0 mm bore. Dorsal root ganglia (