一种中药通过MPP2,9抑制肝细胞癌的侵袭.pdfVIP

Toxicology and Applied Pharmacology 200 (2004) 1 – 6 /locate/ytaap Inhibitory effect of Daesungki-Tang on the invasiveness potential of hepatocellular carcinoma through inhibition of matrix metalloproteinase-2 and -9 activities Ki-Tae Ha, a,b June-Ki Kim, a,b Young-Choon Lee, c and Cheorl-Ho Kima,b,* a National Research Laboratory for Glycobiology, Korean Ministry of Science and Technology, Dongguk University, Kyungju, Kyungbuk 780-714, South Korea b Department of Biochemistry, Molecular Biology and Pathology, College of Oriental Medicine, Dongguk University, Kyungju, Kyungbuk 780-714, South Korea c Faculty of Life Science and Bio-Resources, Dong-A University, Saha-Gu, Busan 608-714, South Korea Received 13 December 2003; accepted 15 March 2004 Available online 18 May 2004 Abstract Daesungki-Tang (DST), a drug preparation consisting of four herbs, that is, Rhei radix et rhizoma (RR; the roots of Rheum coreanum Nakai, Daehwang in Korean), Aurantiii frutus immaturus (AFI; immature fruits of Poncirus trifolita Rafin., Jisil in Korean), Magnoliae cortex (MC; the stem bark of Magnolia officinalis Rehd. Et Wils., Hubak in Korean), and Mirabilite (MS; Matrii sulfas , Mangcho in Korean), is a traditional Korean herbal medicine that is widely used in the treatment of cancer metastasis, gastrointestinal complaints, vascular disorders, and atherosclerosis-related disorders. In this study, water extracts of DST and each of the four ingredient herbs were prepared. The extracts