不同脑温状态下地西泮对大鼠脑缺血组织氨基酸及自由基的影响.docVIP

不同脑温状态下地西泮对大鼠脑缺血组织氨基酸及自由基的影响 不同脑温状态下地西泮对大鼠脑缺血组织氨基酸及自由基的影响 作者：邱小鹰 作者单位：545001柳州，广西医科大学第五附属医院神经内科 【摘要】 目的 探讨不同脑温状态下地西泮对大鼠脑缺血组织谷氨酸（Glu）、Γ氨基丁酸（GABA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSHPx）及丙二醛（MDA）的影响。方法 建立大鼠大脑中动脉缺血再灌注模型，诱导目标脑温，测定轻度高温、常温、亚低温状态下各组脑缺血组织Glu、GABA、SOD、GSHPx及MDA含量。结果 （1）与常温假手术组比较，常温脑缺血对照组及常温地西泮组Glu、MDA水平显著增高（均P0.001），SOD、GSHPx水平显著降低（均P0.001），GABA在常温脑缺血对照组差异无统计学意义（P0.05），在常温地西泮组则显著增高（P0.01）。（2）与常温脑缺血对照组比较，常温地西泮组GSHPx显著增高（P0.001），MDA显著降低（P0.001），而Glu、GABA、SOD差异均无统计学意义（均P0.05）。（3）与常温地西泮组比较，轻度高温地西泮组Glu、MDA显著增高（均P0.001），SOD、GSHPx显著降低（均P0.001），GABA差异无统计学意义（P0.05）；亚低温地西泮组Glu、MDA显著降低（P0.01～0.001），GABA、SOD、GSHPx显著增高（均P0.001）。结论 亚低温状态下，地西泮显著上调GABA水平，有利于地西泮抑制性保护机制的建立，从而增强地西泮的神经保护作用。 【关键词】 脑缺血 脑温 地西泮 氨基酸 自由基 Effects of diazepam under different cerebral temperature on amino acid concentration and oxyradical in cerebral ischemia tissue of rat QIU Xiaoying, WANG He, CHEN Hanming, et al. Department of Neurology, Fifth Affiliated Hospital of Guangxi Medical College, Liuzhou 545001 China Abstract：Objective To reseach the effects of diazepam under different cerebral temperature on the concentration of glutamate（Glu）, Γaminobutyric acid (GABA), superoxide dismutase (SOD), glutathione peroxidase(GSHPX) and malonaldehyde(MDA) in cerebral ischemia tissue of rat. Methods The modle of cerebral middle artery occlusion reperfusion of rats was established to induce the target cerebral temperatue.The concentration of Glu, GABA, SOD, GSHPX and MDA were detected in mild hyperthermia, ordinary temperature and subhypothermia groups respectively. Results (1)Compared with the shamoperation group under normal temperature,the concentration of Glu, MDA had increased obviously in the control group of focal cerebral ischemia and the group using diazepam under normal temperature (all P0.001),but the concentration of SOD, GSHPx had decreased markedly (all P0.001).There was not statistically significant difference in concentration of GABA in contro