中药健身丹对脊髓型颈椎病神经细胞凋亡相关基因表达影响的研究.pdfVIP

∀ 490 ∀ Guizhou Medical Journal, 2009,Vol.33, No.6 中药健身丹对脊髓型颈椎病神经细胞凋亡 相关基因表达影响的研究 * (550002) 徐远坤 陈久毅 通过中药 健身丹 对脊髓型颈椎病神经细胞凋亡相关基因表 影响的研究, 从分子生物学角度探讨中医药防治脊髓型颈椎病的机理 选用SD 大鼠36 只, 体质量(250! 50) g, 随机分组中药治疗组12 只, 空白对照组12 只, 正常对照组12 只采用创新大鼠自体第7颈 椎棘突椎间移植法制造大鼠脊髓压迫模型,并采用改进 Rivlin 的斜板实验检验模型是否成功中药 - 1 - 1 治疗组于造模成功后给予 健身丹 制成粉末悬液灌胃给药2. 66 g ∀ kg ∀ d TU NEL 法神经细 胞凋亡率检测,Bcl2 mRN 测定 相比于空白对照组及正常对照组, 中药治疗组大鼠受压节段 脊髓抗神经细胞抗凋亡基因Bcl2mRN 的表 明显提高, 组织修复增强,神经细胞凋亡减少主观 症状体征观察也显示出相似作用 中药 健身丹 确实能提高大鼠受压节段脊髓抗神经细胞抗 凋亡基因Bcl2mRN 的表 , 促进组织修复, 减少神经细胞凋亡 脊髓型颈椎病 动物模型 健身丹 细胞凋亡 Bcl2 mRN Study on the effects of gene expression on the jianshen pill of chinese medicine to neurocyte apotosis of cervical spondylotic myelopathyX u Yuankuan , Chen J iuy i . D ep artment of Orthop aed ics, Guiy ang Chinese T raditional M edical College , Guiy ang, 550002, Guiz ho u, China. Abstract Objective T o explore the mechanism of cervical spondylotic myelopathy at the level of molecular biology and establish the theory foundation of new medicine development by the studying the effects of the traditional Chinese medicine Jianshen Pill on the cervical spondylotic myelopathy at the level of neurocyte apoptosis Genes. Methods 36 SD rats were divided into 3 groups which were Chinese medicine group(n= 12) , blank control group ( n= 12) , and normal control group ( n= 12) . The blank control groups and the Chinese medicine group will be established as a cervical spondylotic myelopathy rat model, and the normal control group will befed as normal. T he rat models were established by a bone graft from self 7th cervical vertebrae spinous process at the part of C45 or C56 intervertebral space. nd the rat models were checked whether they were succeeded according to the inclined tes