乙酰丙酮比色法测定微量甲醛含量显色条件的研究.pdfVIP

年第 期（总第 期）· ) 54 酿酒科技 2009 3 177 LIQUOR－MAKING SCIENCE ＆ TECHNOLOGY 2009 No．3(Tol．177 乙酰丙酮比色法测定微量甲醛含量显色条件的研究 马美范 （山东轻工业学院食品与生物工程学院，山东 济南 250100 ） ： 摘 要 研究乙酰丙酮比色法测定啤酒中微量甲醛含量的显色条件，考察最大吸收波长、显色剂用量、显色温度和 时间、冷却时间等，确定了最佳试验方案，建立了回归方程y=0.0279x+0.0011 ，其相关系数R=0.9993 。 研究乙酰丙酮 溶液、甲醛标准溶液、甲醛标准使用液放置时间对吸光度的影响，结果表明，乙酰丙酮溶液、甲醛标准溶液5 d 内基 本稳定，而甲醛标准使用液必须现用现配。 ： 关键词 分析方法；甲醛；乙酰丙酮；显色条件 ： ； ： ： （ ） 中图分类号 TS261.7 TS262.5 文献标识码 A 文章编号 1001－9286 2009 03－0054－03 Investigation on the Determination Conditions of Trace Formaldehyde Content by Acetylacetone Colorimetry MA Mei-fan (Food Science and Bioengineering Department,Shandong Institute of Light Industry, Jinan, Shandong 250353, China) Abstract: In this paper, the colorimetric conditions of using acetylacetone colorimetry to determine the content of trace formaldehyde in beer in- cluding the maximum absorption wavelength, the use level of chromogenic reagent, coloration time and temperature, and cooling time etc. were studied. Besides, the best experimental scheme was determined and the regression equation y=0.0279x+0.0011 was established with its correla- tion coefficient R as 0.9993. The effects of the placement time of acetylacetone solution, standard formaldehyde solution and standard formalde- hyde on the absorbance were also studied. The results showed that acetylacetone solution and standard formaldehyde solution kept stable proper- ties within five days, while standard formaldehyde should be formulated immediate