产气荚膜梭菌_2_1毒素融合基因在大肠杆菌中高效表达条件的优化_曾瑾.pdfVIP

800 ( Jiangsu J. of Agr. Sc.i ), 2008, 24( 6) : 800~ 804 B-B 2 1 曾 瑾, 王玉炯, 邓光存, 高蔚丰 ( , 750021) : B -B , BL21( DE ), 2 1 3 B -B , , 2 1 BL21( DE ) ( pXETB2B1) 37 e OD 110 01 1 g /L 5 h, 3 600 , : ; ; + : Q344 . 13 : A : 1000-4440( 2008) 06-0800-05 Optimizing theExpression of theBeta2-toxin andBeta1-toxin FusionGene ofClostrid ium p erf rigens inE. coli ZENG Jin, WANG Y -jiong, DENG G ang-c n, GAO W e-i feng (Key Laboratoryf orProtection and Utilization of Special iolog icalResources in Western China, N ingxia University, Yinchuan 750021, China) Abstract: Based on the expression of B1 tox in and B2 toxin f sion gene ofClostridium p erf rig ens in the recomb inant strain BL21( DE3) ( pXETB2B1) , the expression condition of the f sion proteinwas optmi izedw ith lactose as ind cer. The h ighest expression was obtainedwhen the concentration of lactosew as 011 g/L, ind ction tmi e w as 5 h, cell density of in- d ction w asOD600 110 and the tem perat re was 37 e , respectively. The best w ay to add lactose w as the batch processing. K ey ords: Clostrid ium p erf rigens; f s ion protein; optmi ization of expression cond ition ( Clostridium p efr ringens) , , , , , [ 1] [ 4] , B B IPTG 1 2 IPTG , [ 2, 3] , ,