人参抗应激实验人参对小鼠耐常压缺氧的作用.docVIP

人参抗应激实验：人参对小鼠耐常压缺氧的作用 摘要：目的：掌握小鼠耐常压缺氧的实验方法，观察人参的耐缺氧作用以及对小鼠的抗应激作用。 方法：取小鼠12只，称重，标号，随机分为2组，每组6只。给药组小鼠每只灌胃人参水煎液0.2ml/10g（体重），灌后立即计时。对照组小鼠每只灌胃等容量生理盐水。将广口瓶瓶口涂以凡士林，其中放入钠石灰5g。给药30min后，将小鼠放入广口瓶内，每瓶1只，加盖密封。观察并记录以小鼠呼吸停止作为缺氧死亡时间。汇总全实验室结果，比较给药组与对照组小鼠耐缺氧的时间，进行统计学处理。 结果：人参组与空白对照组相比，人参组的死亡时间明显比较长；结果表明，人参水煎液能明显延长小鼠在缺氧状态下的存活时间，与空白对照组相比，差异有统计学意义。 结论：人参具有明显的抗应激作用, 可以提高小鼠的抗疲劳、耐缺氧能力, 具有抗疲劳和抗应激作用。 Abstract：Objective: The experimental?method?to master?the anti hypoxia,?hypoxia tolerance?in mice to observe?ginseng?and?anti stress effect. Methods:12 mice,?weighing,?grade,?were randomly divided?into 2 groups,?6 rats in each group.?Drug treated mice?per?gavage?ginseng decoction?0.2ml/10g?(weight),immediately?after irrigation.?Control mice?per?gavage?ns.?The?wide mouth?bottle coated with?Vaseline,?which?in?Soda Lime?5G.?After administration of 30min,the mice were put in?a jar,?bottle of 1?only,?stamped seal.?Observe and record the?stop?time of death?as?hypoxia?in mice?breathing.?Summary of?the?laboratory results,?compared?to the?drug group and control group?mice?hypoxia tolerance time,?statistically. Results: Compared with the ginseng group and blank control group, the ginseng group death time was longer; results show, ginseng decoction could significantly prolong the survival time in hypoxia condition, compared with the control group, the difference has statistical significance. Conclusion: Ginseng has obvious anti stress effect, can improve the anti fatigue, hypoxia tolerance, anti fatigue and anti stress effect. 关键词：人参 抗应激 缺氧 前言：人参又称为亚洲参，是具有肉质的根，可药用。人参属于五加科，主要生长在东亚，特别是寒冷地区。33. 【2】王本样. 人参药理学研究的新进展.药学学报1 9 8 0 : 1 5 (5 ): 3 1 2. 【3】杨惠玲, 潘景轩, 吴伟康.高级病理生理学【M』.北京:科学出版社, 19 98 : 17 5 一18 7 . 【4】 韩丽, 王振涛, 申洪超, 等.律复康胶囊对小鼠抗应激能力的影响的实验研究, 中医学报, 2 0 10 , 25 (1 4 6 ) : 9 9 一1 0 0 . 【5】蔡晶, 王万山, 雷林生, 等. 钩吻素子对小鼠抗应激作用的实验研究[ J ] . 广州中医药大学学报, 2007, 24 ( 4 ) : 317. 【6】章观德, 等. 人参的分析. 药学学报1 9 83 ; 1 8 (8 ): 6 0 7.