壳聚糖缓释膜的制备及性能研究.docVIP

壳聚糖缓释膜的制备及性能研究 壳聚糖缓释膜的制备及性能研究 作者：季娟娟 丁仲鹃 杨雪莲 作者单位：昆明医学院附属口腔医院 口腔修复科，云南 昆明 650031 　　【摘要】 目的 探索壳聚糖膜的制备及该膜的缓释、降解性能，并研究该膜对成骨细胞的毒性作用。方法 以溶胀度为评价指标，设计正交实验，确定制备壳聚糖膜的最佳参数。检测该膜对牛血清白蛋白的释放率及膜在溶菌酶作用下的降解率。使用噻唑蓝（MTT）法检测该膜对成骨细胞的毒性。结果 当稀醋酸浓度为1％、壳聚糖浓度为 2 mg/mL、三聚磷酸钠浓度为6%、交联时间为1 h时，膜的溶胀度最小。该膜8 d时释放率达33.13％，4周内可降解36.73％。该膜对胎鼠成骨细胞的毒性作用为0级或1级。结论 用最佳参数制备的膜具有良好的缓释能力、可降解性，对胎鼠成骨细胞无毒性。 　　【关键词】 壳聚糖膜 缓释 降解 成骨细胞 细胞毒性 　　[Abstract] Objective To develop a best chitosan film for using as a drug sustained-release system through the evaluation of the sustained-release property, degradation property, and cytotoxicity to osteoblast. Methods Orthogonal experiments were designed to determine the best combination of chitosan film preparations. Drug release rate was determined with Coomassie brilliant blue G250. In a separate study, chitosan films were placed into the test tubes with buffer solution and 107 U/L lysozyme. The degradation rate was calculated. Osteoblasts derived from fetal rat calvarial were cultured on chitosan films. Cell proliferation was tested by methyl thiazolyl tetrazolium（MTT） assay.The relative growth rate was calculated and the cytotoxicity was graded. Results The best processing condition was 1% acetic acid, chitosan concentration of 2 mg/mL, 6% sodium tripolyphosphate（STPP） concentration, and cross-linking time of one hour. The resulting chitosan film released 33.13％ of bovine serum albumin（BSA） within 8 d,36.73% of BSA within four weeks and the cytotoxicity grade was 0 or 1. Conclusion This chitosan film possesses good sustained release property, and a good degradation rate. 　　[Key words] chitosan film； drug sustained-release； degradation； osteoblast； cytotoxicity 　　在钛种植体表面附着促骨生长因子可加速种植体的早期愈合，提高骨结合率，但由于生长因子是多肽类物质，其应用受到很多限制。因此需要引入载体或缓释系统，使其既能保护生长因子的生物活性，又能在一定时间内持续释放生长因子促进新骨的形成。壳聚糖以其良好的生物相容性、可降解性、黏附性[1-3]等优点成为开发研制的热点。本实验采用溶剂蒸发法制备壳聚糖膜，并选用无生物毒性的三聚磷酸钠对膜进行物理交联，制备适合蛋白质药物缓释的壳聚糖膜，对影响膜制备的主要参数进行探索，并研究该膜的降解性、缓释性和细胞毒性。 1 材料和方法 　1.1 材料 　　SD胎鼠