应激性高血压大鼠脑干及下丘脑垂体肾上腺轴中肾上腺髓质素及其受体mRNA表达的改变.pdfVIP

Acta Physiologica Sinica , December 25, 2004, 56 (6): 723-729 723 Research Paper Changes of adrenomedullin and its receptor components mRNAs expression in the brain stem and hypothalamus-pituitary-adrenal axis of stress-induced hypertensive rats LI Xia, LI Liang**, SHEN Lin-Lin, QIAN Yuan, CAO Yin-Xiang, ZHU Da-Nian* Department of physiology and pathophysiology, Shanghai Medical Chollege of Fudan University, Shanghai 200032, China Abstract: In this study, reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the changes in mRNAs levels of preproadrenomedullin (ppADM) gene encoding adrenomedullin (ADM) and the essential receptor components of ADM, calcitonin receptor-like receptor (CRLR), and the receptor activity modifying protein 2 and 3 (RAMP2 and RAMP3) in the medulla oblongata, hypothalamus, midbrain, pituitary gland and adrenal gland of the stress-induced hypertensive rats. It was shown that chronic foot- shock and noise stress for 15 consecutive days induced a significant increase in systolic blood pressure (SBP) and unique changes in ppADM and its receptor components mRNAs in all areas studied. As compared with the control group, the level of ppADM mRNA, normalized against a glyceraldehydes-3-phosphate dehydrogenase (GAPDH) control, was up-regulated in the hypothalamus-pitu- itary-adrenal (HPA) axis, but down-regulated in the medulla oblongata and midbrain (P0.01 and P0.05, respectively). The relative amount of CRLR mRNA was higher in the hypothalamus than that in other areas. The level of CRLR mRNA expression was significantly increased in the medulla oblongata of the stress group (P0.01), but decreased in the midbrain (P0.01) as well as hypothalamus(P0.05), as compared with that of the control group. Chronic stress for 15 consecutive days produced an increase in the level of RAMP2 mRNA expression in the medulla oblongata (P0.01) and a decrease in