姜黄素对人肝细胞HepG2中低密度脂蛋白受体基因表达影响的研究.pdfVIP

HepG2 1, 2 2* 2 2 2 2 2 窦晓兵 , 沃兴德 , 范春雷,俞颖 ,钱颖 , 黄小黎,凌霞 ( 1 , 100029; 2 , 310053) : 研究不同浓度姜黄素对人肝细胞 HepG2中 源低密度脂蛋白受体 (LDLR)表达的影响, 在受体水平上探讨姜黄 素的调脂作用机制 以荧光试剂标记配体法,利用流式细胞仪技术和荧光显微镜技术研究姜黄素对H epG2细胞 LDLR 表达活性的影响利用细胞免疫技术结合荧光显微镜技术流式细胞术研究姜黄素对HepG2细胞 LDLR表达数量的影响 姜黄素可显著增加人肝细胞HepG2 源 LDLR的表达 (P 005) 姜黄素能够通过增加人肝细胞 HepG2 LDLR 的表达起到调节血脂和抗动脉粥样硬化的作用 : 姜黄素;低密度脂蛋白受体;H epG2;流式细胞术 : R965 : A : 1001- 2494( 2007) 08- 0572- 04 Effect of Curcum in on the Expression of Low Density Lipoprotein Receptor in HepG2 Cell Line 1, 2 2* 2 2 2 2 2 DOU Xiaobing ,WO Xingde , FAN Chunlei, YU Ying, QIAN Ying, HUANG Xiaoli, LING X ia ( 1B eij ing Un ivers ity of Trad itional Ch ineseM edicine, B eij ing 100029, China; 2L if e S cience College, Zhej iang Ch ineseM ed ical University, H ang zho 310053, China) ABSTRACT: OB ECTIVE To investigate the influenceofcurcum in on the endogenous low density lipoprotein receptor (LDLR) ex pression in thehuman hepatoma cell lineHepG2 and seek for the lipidsregulatingmechanism of curcuminM ETHODS The ligands ofLDLR and LDLwere labeled by a fluorescent reagent andDiIThe effect ofcurcum in on the expression of functionalLDLR inHepG2 cell linewasmeasured by FluoM icroscopy( FM) and Fluorescence Flow CytometricMethods( FFCM) The effect of curcumin on the numbers ofLDLR expression in theHepG2wasmeasured by FM, ELISA and FFCMRESULTS Curcum in obviously upregulated the expression of LDLR inHepG2 (P 005) CONCLUSION Curcumin is apotent activatorofLDLR expression Itmaybe one of its mechanisms tomodulate the plasma LDLC by increasing the expression ofLDLR in Hepatocells KEY WORDS: curcum in; low density lipoprotein receptor; HepG2; flow cytometry ( low density lipoprotein re LDLR ceptor, LDLR), , , HepG2, LDLR LDL( LDL ,,