Bacterial+and+archaeal+community+structures+in+the+Arctic+deep-sea+sediment.pdfVIP

Acta Oceanol. Sin., 2015, Vol. 34, No. 2, P. 93–113 DOI: 10.1007/s13131-015-0624-9 E-mail:hyxbe@263.net Bacterial and archaeal community structures in the Arctic deep- sea sediment 1, 3 2 1 1 1 1 1 LI Yan , LIU Qun , LI Chaolun , DONG Yi , ZHANG Wenyan , ZHANG Wuchang , XIAO Tian * 1 Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 2 State Field Research Station of Jiaozhou Bay Marine Ecology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 3 Center for Transport Phenomena, Energy Research Institute, Shandong Academy of Sciences, Jinan 250014, China Received 3 March 2014; accepted 18 July 2014 ©The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg 2015 Abstract Microbial community structures in the Arctic deep-sea sedimentary ecosystem are determined by organic matter input, energy availability, and other environmental factors. However, global warming and earlier ice-cover melting are affecting the microbial diversity. To characterize the Arctic deep-sea sediment microbial diversity and its rela- tionship with environmental factors, we applied Roche 454 sequencing of 16S rDNA amplicons from Arctic deep-sea sediment sample. Both bacterial and archaeal communities’ richness, compositions and structures as well as tax- onomic and phylogenetic affiliations of identified clades were characterized. Phylotypes relating to sulfur reduction and chemoorganotrophic lifestyle are major groups in the bacterial groups; while the archaeal community is domi- nated by phylotypes most closely related to the ammonia-oxidizing Thaumarchaeota (96.66%) and methan