癫疒间大鼠海马齿状回微管相关蛋白2表达规律及地西泮干预的研究.docVIP

癫疒间大鼠海马齿状回微管相关蛋白2表达规律及地西泮干预的研究 癫疒间大鼠海马齿状回微管相关蛋白2表达规律及地西泮干预的研究 作者：王胜军，迟兆富，陈雯，赵彦新，迟令懿 作者单位：250012济南，山东大学齐鲁医院神经内科（王胜军,迟兆富,陈雯），神经外科（迟令懿）；济南市中心医院神经内科（赵彦新） 【摘要】 目的 研究致疒间大鼠神经元树突标记物微管相关蛋白2（MAP2）的表达规律、与癫疒间发生的关系及在地西泮干预下的变化。方法 建立戊四氮致疒间大鼠模型，分为戊四氮生理盐水组、戊四氮地西泮组、地西泮戊四氮组及正常对照组。采用免疫组化方法观察大鼠疒间后海马结构MAP2免疫反应性(MAP2IR)，及其在地西泮干预下的动态变化，用平均吸光度值(COD)表示免疫反应性的强度。结果 戊四氮生理盐水组及戊四氮地西泮组海马齿状回分子细胞层MAP2 IR的COD值于疒间后3 d增强(均P005)，7 d明显增强(均P001)，14 d达到高峰(均P001)，28 d降至正常对照组水平；地西泮戊四氮组未能观察到MAP2的明显变化。结论 致疒间大鼠海马齿状回MAP2IR变化显著，疒间后的地西泮干预不能改变MAP2IR的变化规律。 【关键词】 癫疒间；微管相关蛋白；地西泮；戊四氮 　　Changing features of microtubule associated protein 2 expression in hippocampal dentate gyrus of epileptic rats and the intervention results after using Diazepam WANG Shengjun, CHI Zhaofu, CHEN Wen, et al.　Department of Neurology, Qilu Hospital of Shandong University, Jinan 250012, China 　　Abstract：Objective To investigate the changing features of microtubule associated protein 2 (MAP2) expression, which was a neural dendrite marker, of epileptic rat and the intervention results after using Diazepam. The relations between MAP2 and epileptogenesis were also explored.Methods Model of epileptic rat was established by Pentylenetetrazol (PTZ) and divided into PTZNA group,PTZDiazepam group, DiazepamPTZ group and normal control group. Immunohistochemistry method was applied on hippocampus of epileptic rats to determine the change of MAP2 immunoreactivity (MAP2IR) with or without Diazepam intervention at various time points. MAP2IR was showed by mean optical density (COD).Results In the PTZNA group and PTZDiazepam group， MAP2IR in molecular cell layer of hippocampal dentate gyrus increased after 3 days (all P005), boosted obviously after 7 days (all P001)，reached peak after 14 days (all P001) and came back to normal level after 28 days of epilptogenesis. In contrast, no marked change was observed in DiazepamPTZ team. Conclusions Marked changes of MAP2IR are found in hippocampal dentate