芹黄素对老年痴呆小鼠学习记忆能力的影响.pdfVIP

第21卷第3期 广摇 东摇 药摇 学摇 院摇 学摇 报 Vol.21No.3 2005年6 月 JOURNAL OF GUANGDONG COLLEGE OF PHARMACY Jun.2005 芹黄素对老年痴呆小鼠学习记忆能力的影响 1 2 3 3 赵宇红 ,陈伟强 ,罗少洪 ,杨摇 红 (广东药学院1.药理教研室;2.病理生理教研室;3.生物化学教研室,广东广州510240) 摘要:目的研究芹黄素对老年痴呆(AD)小鼠学习和记忆能力的改善作用。 方法 取10 个月龄的小鼠,以D鄄半乳糖和AlCl 复制老年3 痴呆动物模型,建模成功的AD小鼠用不同浓度的芹黄素腹腔注射,利用Y鄄型迷宫进行被动回避反应实验和信号方位辨别反应实验, 测试小鼠学习记忆能力及信号方位辨别能力改变。 结果腹腔注射芹黄素的小鼠学习能力和记忆保持率均显著优于模型对照组(P0. 01)。 结论 芹黄素能有效改善老年痴呆症小鼠的学习和记忆能力。 关键词:芹黄素;老年痴呆症小鼠;D鄄半乳糖;学习;记忆 中图分类号:R961摇 文献标识码:A摇 文章编号:1006-8783(2005)03-0292-03 Effect of apigenin on learningandmemorybehaviorinmicewith alzheimer爷sdiseaseinducedwithD鄄 galactose 1 2 3 3 ZHAO Yu鄄hong , CHEN Wei鄄qiang , LUO Shao鄄hong , YANG Hong (1. Department of pharmacology,2. Department of Pathophysiology,3.Department of Biochemistry,Guangdong College of Pharmacy,Guangzhou,Guangdong 510240) Abstract:Objective To study the effects of apigenin on mice model with Alzheimers disease induced by D鄄galactose its mechanisms. MethodsThe mice model with Alzheimers disease was induced by subcutaneous injection of D鄄galactose in the nape of ten month Kunming mice. Apigenin were administered in different dose. Y鄄maze trial was performed to evaluate their learning and memory behaviors. Results Compared with the D鄄galactose induced mouse model, apigenin could improve the ability of escaping electrical stimulation significantly(P0.01). Conclusion The results suggested that apigenin might be beneficial to improving the learning and memory behaviors in micewith Alzheimers disease induced by D鄄galactose. Key words:apigenin;aging mice;D鄄galactose;learning;memory 摇 摇 近年来国内外对芹黄素的研究发现,其与雌激素 sigma公司;其他试剂均为分析纯。 结构相似,具有类雌激素和抗雌激素的双重活性,对雌 1.1.3摇 MG鄄3 型Y迷宫为三兴声电公司产品。 激素相关的疾病,如乳腺癌、子宫内膜癌等具有良好疗 1.2摇 方法