2009.047.谷胱甘肽(GSH)对牛卵母细胞体外受精胚胎发育影响.pdfVIP

, Jo rnal ofAnh iAgr.i Sci. 2009, 37( 33): 16398, 16404 李菲菲 张士敏 ( GSH) 1 1 2 3 魏红芳 , 张长兴 , 昝林森 , 徐照学 ( 1. , 450011; 2. , 712100; 3. , 450002) [目的]研究谷胱甘肽( GSH ) 对牛体外受精胚胎发育的影 [方法]通过对牛卵母细胞进行体外受精和受精卵体外培养, 研究 了谷胱甘肽( GSH ) 对牛胚胎体外发育的影 [结果]添加谷胱甘肽组的桑囊率分别比对照组高 3. 34%和 2. 26%, 囊胚发育率分别比 对照组高 4. 57% 和 4. 05%, 但差异不显著(P 0. 05)[结论] GSH在牛体外受精的胚胎发育过程中有一定的保护和促进作用 牛; 体外受精; 谷胱甘肽( GSH ) S823 A 0517- 6611( 2009) 33- 16398- 01 Effect of GSH on theD evelopm ent of IVF Embryos of BovineOocytes WEI Hong-f ng et l ( Zhengzho College ofAnmi alH sbandry and Engineering, Zhengzho , H enan 450011) Abstr ct [ Objective] The research ami ed to st dy the effect of GSH on the developm ent of bovine in v itro fertilization ( IVF) embryos. [Method] Thro gh IVF and in v itro c lt re of zygote, the effects ofGSH on the development of bovine embryos in v itro were st died. [ Res lt] Themor la rate in the gro ps of add ingGSH w ere higher 3. 34% and 2. 26% resp. than control gro p. The blastocyst developm ent rateswere h igher 4. 57% and 4. 05% than control gro p, w itho t sign ificant difference (P 0. 05). [ Concl sion] GSH had certain protective and pro- m otion effects on the development of bovine IVF embryos. Key words Bovine; IVF; GSH (GSH), - ( COC) 2~ 3, ,, , 3, 10~ , 20/( 90 Ll) , 90 L l 10 Ll, GSH CO 6~ 8 h 2 [ 1- 2] ( IVF) 1. 4 10 ~ 20 , /100 Ll, 24 h, 1 IVF 267(h) 1. 1 , , 2 , , ,