18β-GA对人高转移卵巢癌HO-8910PM细胞增殖、黏附和侵袭能力的影响.pdfVIP

18 13一GA对人高转移卵巢癌HO一8910PM细胞 增殖、黏附和侵袭能力的影响 陈杰1，庞江琳2，覃燕梅1，梁念慈1 (1广东医学院，广东湛江524023；2广东医学院附属医院) 测HO-8910PM细胞增殖抑制率；细胞黏附试验检测细胞黏附能力；TransweUchamber法检测细胞侵袭能力；Western 黏附和侵袭能力；Westernblot法检测表明药物能下调FAK、MMP-9蛋白的表达。结论1813一GA可抑制HO一 8910PM细胞黏附、侵袭，其机制可能是通过下调FAK、MMP-9蛋白表达而进行的。 关键词：t813一甘草次酸；黏附斑激酶；基质金属蛋白酶-9；卵巢肿瘤，卵巢癌 中图分类号：R737．31文献标志码：A 文章编号：1002-266x(2011)474)012-03 inHO-8910PMcells Effectsof acidonthe andinvasion 18p—glycyrrhetinicproliferation，adhesion Nian-ci CHEN觑‘，PANG Yan—mei，HANG Jiang·lin，Qm Medwal (1GuangdongCollege，Zhanjiang524023，P．R．China) To theeffectsof the Abst聃ct：Objective proliferation，adhesion， investigate 18B—glycyrrhetinicacid(18B-GA)on invasionandthe of ofhuman metastaticovariancarcinonlaHO-8910PMcells．Methods FAK，MMP-9 expression highly HO-8910PMcellsweretreatedwith MIF wasusedtodeterminethe 18B．GA，then inhibition；Transwell assay growth chamberWaS todeterminetheeffectoninvasionofthecells adhesionwas assay performed by1813-GA；cellassayperformed todetecttheadhesionof blot was todetectthe ofFAKand HO-8910PM；Western protein analysisperformed expreesion inhibitedthe in also MMP-9．Results 18B．GA growth，proliferation，adhesion，invasioncells；1813·GAdown-regulated the of caninhibitthe andinvasionofHO- FAK，MMP-9 proliferation，adhesion expression protein．Conclusion1813-G