2种猪圆环病毒2型TaqMan实时荧光定量PCR检测方法的建立及比较（英文版）.pdfVIP

Agricultural Science Technology, 2015, 16(1): 3-8 Copyright 訫 2015, lnformation lnstitute of HAAS. All rights reserved Molecular Biology and Tissue Culture Establishment and Comparison of Two TaqMan Real-time PCR Methods for PCV2 1, 2 1 1 1 1 1 Zhongtao ZHOU , Xiaomin WANG , Wei WANG , Aihua MAO , Libin WEN , Yanxiu Nl , Kong- wang HE1 * ’ 1. Ministry of Agriculture s Key Laboratory of Veterinary Biologicals Engineering and Technology, National Center for Engineering Research of Veterinary Biological Products, lnstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China 2 种猪圆环病毒 2 型 TaqMan 实时荧光定量 PCR 检测方法 Abstract [Objective] ln this study, the quantitive detection of PCV2 (porcine cir- 的建立及比较 covirus type 2) in vitro was achieved. We aimed to establish two kinds of TaqMan real-time PCR methods based on PCV2 ORF1 and ORF2 respectively and compare 周忠涛 1, 2，王小敏 1，汪伟 1，茅爱华 1，温立斌 1 ， them. [Method] According to the relatively conserved sequences of PCV2 ORF1 and 倪艳秀 1 ，何孔旺 1* （1.江苏 省农 业科学 院兽 ORF2 registered in GenBank, two pairs of specific primers and TaqMan probes were designed and synthesized. Then the recombinant plasmids containing the 医研究所 ，农业部兽用生物制品工程技术重点 whole sequences of PCV2 ORF1 and ORF2 were constructed to draw the standard 实验室 ， 国家兽用生物制品工 程技 术研 究中 curves through optimizing the re