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Chem.Res.Chin.Univ.,2014,30(5),778—784
A
NovelThermostable from
ff-Galactosidase
Geobacillus HTA42
kaustophilus
YU
Shanshanl,YIN Lil,XUChunchunl,LI
Hongbin92,WANGXinyin91,FENG Jin91,
HAN andLIU
Hongxian93Shuyin91’4+
,.Jilin
GinsengAcademy,2.AffiliatedHospital,ChangchunUniversityofChineseMedicine,
13011
7,PR.China;
Changchun
3.Jilin
AgriculturalUniversity,Changchun130118,PR.China;
Center Institute
4.ChangchunofMassSpectrometry,ChangchunofAppliedChemistry,
ChineseAcademic R.China
130022,P
ofScience,Changchun
AbstractAnovel as the bacterium
GkGallA,from
thermostable口-galactosidasegene,designmed thermophilic
Geobacillus HTA426was
clonedandheterol020uslv inEscherichia
kaustophilus overexpressed coli(E.coli).Based
onthe tothe 1 thatwasthe of
sequenceanalysis.GkGallAbelongsglycosylhydrolasefamily first口.galactosidase
bacterial usinthis molecularofGkGallAdeterminedsodium
originsexpressedby family.TheaDparent weight by
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