杨树对潮霉素敏感性研究.pdfVIP

吉林农业大学学报 2010, 32( 1) : 47 ~ 50 http : xuebao.j lau. edu. cn Journal of Jilin Agricultural University Email :j lndxb@ vip . sina. com 王 丹, 邹 莉 , 王 义, 杨民宝 东北林业大学林学院, 哈尔滨150040 : 以山新杨欧美杨小黑杨无菌苗的幼叶叶片分化的不定芽茎段为外 体, 研究了其对潮霉素的敏 感性结果表明: 叶片分化时山新杨和欧美杨的潮霉素敏感性为20 mg/ L, 小黑杨为30 mg/ L; 不定芽生长 和生根时山新杨和小黑杨的敏感性为20 mg/ L, 欧美杨为10 mg/ L; 茎段生长和生根时山新杨和小黑杨的敏 感性为60 mg/ L, 欧美杨为20 mg/ L : 山新杨; 小黑杨; 欧美杨; 潮霉素; 离体培养; 选择压力 : S79211 : A : 2010) Study on the Sensitivity of Poplar to Hygromycn WA NG Dan, ZOU Li, WA NG Yi, YA NG Minbao Forestry School of Northeast Forestry University , H arbin 150040, China Abstract: Leaves, buds and stems o Pop ulus davidiana P . bollena , Pop ulus euramericana and Pop ulus simonii P. nigra were cultured as explants to investigate in luence o hygromycin. T he results showed that the optimal concentrations o hygromycin to Pop ulus davidiana P . bollena and Pop ulus euramericana were 20 mg/ L and to Pop ulus simonii P . nigra was 30 mg/ L at the stage o lea di erentiation; 20 mg/ L to Pop ulus davidiana P. bollena and Pop ulus simonii P . nigra and 10 mg/ L to Pop ulus euramericana at the stage o bud growing and rooting; 60 mg/ L to Pop ulus da vidiana P . bolleana and Pop ulus simonii P. nigra and 20 mg/ L to Pop ulus euramericana at the stage o stem growing and rooting. Key words: Pop ulus davidiana P . bollena; Pop ulus simonii P. nigra; Pop ulus euramericana ; hygromycin ; culture in vitro; select ive pressure , , , , , B ( Hygromycin , B) , ,