《Arabidopsis AtADF1 is Functionally Affected by Mutations on Actin Binding Sites》.pdfVIP

Journalof Plant 201 Biology3，55(3)：250-261 Integrative F。e孰A噍iel@ R．eSr@a AtADFlis Affected by ArabidopsisFunctionally MutationsonActin Sites Binding Chun-Hal andJia—YaoLiu Dong‘。Wei-PingTang 2661 ofLife 09，China CollegeSciences，QingdaoAgriculturalUniversity，Qingdao author ’Corresponding Tel／Fax：+865328608 0640；E-mail：chunhai79@yahoo．com onlineon29November2012at and Available www．wileyonlinelibrary．com／joumal／jipb www．jipb．net doi：10．111l／jipb．12015 Abstract The actin tobothmonomericandfilamentousis factor(ADF)binds actin，and plant depolymerizing betterunderstandtheactin sites involvedinthe ofactinfilaments．To directly depolymerization binding functions ofthe thaliana mutantsofAtADFland their ArabidopsisL．AtADFl。wegenerated investigated ／nvitroand／n ofmutants aminoacidsubstitutionsrevealedthat vivo．Analysis harboring charged and the and anactin site withthe at a-helix3 residues(Ar998Lysloo)located forming bindingtogether N-terminus areessentiaIforbothG-a