《Arabidopsis AtADF1 is Functionally Affected by Mutations on Actin Binding Sites》.pdfVIP

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《Arabidopsis AtADF1 is Functionally Affected by Mutations on Actin Binding Sites》.pdf

Journalof Plant 201 Biology3,55(3):250-261 Integrative F。e孰A噍iel@ R.eSr@a AtADFlis Affected by ArabidopsisFunctionally MutationsonActin Sites Binding Chun-Hal andJia—YaoLiu Dong‘。Wei-PingTang 2661 ofLife 09,China CollegeSciences,QingdaoAgriculturalUniversity,Qingdao author ’Corresponding Tel/Fax:+865328608 0640;E-mail:chunhai79@yahoo.com onlineon29November2012at and Available www.wileyonlinelibrary.com/joumal/jipb www.jipb.net doi:10.111l/jipb.12015 Abstract The actin tobothmonomericandfilamentousis factor(ADF)binds actin,and plant depolymerizing betterunderstandtheactin sites involvedinthe ofactinfilaments.To directly depolymerization binding functions ofthe thaliana mutantsofAtADFland their ArabidopsisL.AtADFl。wegenerated investigated /nvitroand/n ofmutants aminoacidsubstitutionsrevealedthat vivo.Analysis harboring charged and the and anactin site withthe at a-helix3 residues(Ar998Lysloo)located forming bindingtogether N-terminus areessentiaIforbothG-a

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