HPLC法测定不同产地博落回中白屈菜红碱和血根碱含量.pdf

# 434# 安 徽 医 药 AnhuiM edical and PharmaceuticalJournal 2011 Apr; 15( 4) HPLC法测定不同产地博落回中白屈菜红碱和血根碱含量 1 1 2 3 , , , ( 1. 安徽中医学院, 安徽合肥 230031; 2. 安徽省肺科医院, 安徽 合肥 230038; 3. 安徽省医学科学研究院, 安徽合肥 230061) : HPLC H PLC , : SH I- MADZU C ( 4. 6 mm @ 250 mm, 5Lm ), : 0. 4% - (73B27), 1. 2 m l# m i - 1, 30e 18 98. 26% , 97. 73% ; RSD 1. 87% , 1. 81% , , : ; ; ; Determ ination of chelerythrine and sanguinnarine inmacleaya cordata from the different areas by HPLC 1 1 2 3 WANG Shu-gua g, HUA Pe-i li g , WANG H a -lei, HU Hu-i pi g (1. Anhui College of Trad itional ChineseM edicine, H ef ei,Anhui 230031; 2.AnhuiLung H osp ital, H ef ei,Anhui 230038; 3. Anhui Institute of M edical Sciences, H ef ei,Anhui 230061) Abstract: A im To develop a H PLC m ethod for the determ i atio of chelerythri e a d sa gui air e i m acleaya cordata from d iffere t p lace of productio . M ethods H PLC w as adopted, SH IMADZU C18 colum ( 4. 6 mm @ 250 mm, 5Lm ) w ith a mobile phase aceto itrile - 0. 4% (V /V ) phosphoric acid solutio ( 27B73) by diode array detector at 270 m. R esults The average recoveries of chelerythri e a d sa gui ari cw ere 98. 26% , 97. 73% , respectively. RSD w ere 1. 87% , 1. 81% respectively. Conclusion The method is se sitive a d re- liable a d ca be used as a quality co trolm ethod of the cheleryth ri e a d sa gu i air e i total alkaloid ofm acleaya cordata. K ey words: m acleaya cordata i differe t areas; chelerythri e; sa gu i air e; H PLC Macl