不同产地及外观形态夏枯草中齐墩果酸和熊果酸的含量比较.pdf

广东药学院学报 摇 256 Journal of Guangdong Pharmaceutical College摇 Jun.2009,25(3) 摇 不同产地及外观形态夏枯草中齐墩果酸和 熊果酸的含量比较 1 2 3 蔡中齐 ,马鸿雁 ,张勉 (1. 浙江医药高等专科学校,浙江 宁波315100;2.广东药学院 中药学院,广东 广州510006; 3.中国药科大学 生药学研究室,江苏 南京210038) 摘要:目的 比较不同产地、长度、色泽的夏枯草中齐墩果酸和熊果酸的含量,为更全面地控制夏枯草的 质量提供依据。 方法采用高效液相色谱法,以Shim鄄PackC 为色谱柱,乙腈鄄甲醇鄄水鄄乙酸铵(体积比68 18 颐16颐16颐0.5)为流动相,检测波长为215 nm,流速为0.8 mL/ min。 结果与结论 不同产地夏枯草中齐墩 果酸和熊果酸的含量差异较大,果穗短者两者含量高于果穗长者,紫红色果穗者两者含量高于棕色果穗。 关键词:夏枯草;产地;果穗;齐墩果酸;熊果酸 中图分类号:R284.2摇 文献标识码:A摇 文章编号:1006-8783(2009)03-0256-03 Content analysis of oleanolic acid and ursolic acid in Prunella vulgaris based on its provenances and appearances 1 2 3 CAI Zhong鄄qi ,MA Hong鄄yan ,ZHANG Mian (1. Zhejiang Pharmaceutical College,Ningbo,Zhejiang 315100,China;2. Guangdong Pharmaceutical College,Guangzhou,Guangdong 510006,China;3. Department of Pharmacognosy,China Pharmaceutical University,Nanjing,Jiangsu210038,China) Abstract:Objective To compare the contents of oleanolic acid and ursolic acid in Prunella vulgaris with different appearances and from different provenances,in order to control the quality of Prunella vulgaris. Methods Sampleswere analyzed on a Shim鄄Pack C column. The mobile phase consisted of acetonitrile鄄 18 -1 methol鄄water鄄ammonium acetate (68颐16颐16颐0.5) under a flow rate of 0.8 mL ·min . The detection wavelength was set at 215 nm. Results and Conclusions There were large differences in contents of oleanolic acidandursolicacidamongPrunellavulgariswithdifferentprovenancesandappearances,higher in Prunella vulgariswithshortandmauveears. Thismethodwassuitableforthequality controlofPrunella vulgaris. Key words:Prunella