不同产地掌叶大黄HPLC指纹图谱的比较.pdf

中草药 　Chinese Traditional and Herbal Drugs 　第 34 卷第 5 期 2003 年 5 月 ·457 · References : [ 1] 　Xing W X , Bi H M , Tian L , et al RAPD (random amplified polymorp hic DNAs ) technology and application of RAPD in p harmacognosy study [J ] Pharm J Chi n PL A (解放军药学学 ) ( ) 报 , 1999 , 15 3 : 2528 [2 ] 　Cox A V , Bennett M D , Dyer T A U se of t he polymerase chain reaction to detect spacer size heterogeneit y in plant 5S rDNA 图3 　PCR 扩增谱带 gene cluster s and to locate such cluster s in wheat ( Triticu m aestiv u m L ) [J ] Theor A pp l Genet , 1992 , 83 : Fig3 　PCR extension pattern 684690 需D NA 量少 , 是一种分子水平上鉴定体细胞杂种 [ 3 ] 　Zanke C , Borisj uk N , Ruoss B , et al A specific oligonucleotide 的好方法 ,但此法检测的基因位点数 目较少 , RAPD of t he 5S rDNA spacer and speciesspecific element s identify symmet ric somatic hybrids between S olan u m t uberos u m and S 技术无需专门设计 RAPD 扩增反应的引物 , 也无需