半夏蛋白抗肿瘤活性组分的提取分离.pdf

R284A 1005-5304(2007)01-0045-03 FU Yun, HUANG Bi-sheng, LI Juan, et al (Hubei College of TCM, Wuhan 430061, China) To isolate the anti-tumor protein groups from Pinellia ternata rhizome and investigate the anti-tumor activity of these protein groups on Bel-7402 cell.The total raw protein was isolated with sepharose column chromatography. Methyl-thiazolyl-tetrazolinm (MTT) was used to analyze the effect of Pinellia ternata Protein on inhibiting growth of tumor cells. Apoptosis was detected by flow cytometry (FCM). The 30% and 60% (NH ) SO deposition part of total proteins from Pinellia 4 2 4 ternata rhizome have no certain relationship between quantity and inhibitory action, but protein peak 3 eluted with 0.05 mol/L or 0.1 mol/L NaCl from 30% (NH ) SO deposition part showed a effect of 4 2 4 concentration depending. FCM analysis showed that the protein of 30% (NH ) SO deposition part could 4 2 4 induce apoptosis. The 30% (NH ) SO deposition part of total proteins from Pinellia ternata 4 2 4 rhizome could significantly inhibit Bel-7402 growth and induce its apoptosis. Pinellia ternataBel-7402anti-tumor protein groupsassay of anti-tumor activity