酪蛋白肽对小鼠抗氧化酶作用的研究.pdfVIP

242 Acta Nutrimenta Sinica,Jun.,2010, Vol.32 No.3 酪蛋白肽对小鼠抗氧化酶作用的研究 陈东平，牟光庆 (大连工业大学 生物与食品工程学院，大连 116034) 【摘 要 】 目的 研究不同分子量范围酪蛋白肽对小鼠肝脏组织中抗氧化酶活性的影响。 方法 将 48 只雌雄各半的 昆明种小鼠，平均分成肽分子量大于 3ku、1k~3ku、小于 1ku 和对照四个组。样品组每天灌胃 10mg/ml 多肽溶液 0.2ml， 对照组灌胃蒸馏水。各组随机选取 6 只，分别灌胃 15d 和 30d，断颈处死后取其脾脏和肝脏进行抗氧化指标测定。结 果 酪蛋白肽能够使小鼠肝脏组织中谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）活性增强，使丙二醛 （MDA）含量下降，且灌胃 15d 与 30d 时抗氧化酶活性差异显著。结论 酪蛋白肽可增强小鼠抗氧化性作用，肽分子 量 1ku 以下对小鼠抗氧化酶系作用显著。[营养学报，2010，32（3）：242－244] 关键词：酪蛋白肽；GSH-P ；SOD ；MDA X 中图分类号： 文献标识码：A 文章编号：0512-7955(2010)-03-0242-03 EFFECTS OF CASEIN PEPTIDES ON ANTIOXIDATIVE ENZYMES IN MICE CHEN Dong-ping, MU Guang-qing （ ） School of Biology and Food Engineering, Dalian Polytechnic University, Dalian 116034 China 【Abstract 】Objective To study the antioxidant effects of casein peptides with different molecular weights on liver tissue in mice. Methods Forty-eight mice were divided into four groups, and fed ig casein peptides in different molecular weights, 1ku, 1-3ku, 3ku and distilled water as control. Results Casein peptides could improve the antioxidation ability of the mice, as to increase glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities of liver tissue, and reduce malondialdehyde (MDA). The antioxidant enzymes activities after giving casein peptides for 30d and 15d were significantly different. Conclusions Casein peptides can enhance the antioxidation of the mice and especially more effect with the molecular weight 1000u. [ACTA NUTRIME