脐血清用于CIK诱导及细胞培养的观察.pdfVIP

144 ( J YM C) 2003 6 , 22( 3) : 144 [ ] 1006- 9674( 2003) 03- 0144- 04 CIK 黄 玲, 张有顺, 王德山, 程范军 ( , 442008) [ ] 目的: A B PBMC CIK 方法: AB ( 10 ) CIK , 3 CIK ; CIK CD3CD56; H- T dR CIK SMMC- 7721B l- 7402H p- 3b, HIC- 25 1, L- 02, K562 K562 结果: CIK + + , 64. 3 67. 4 ( t = 1. 003, P 0. 05) , CD3 CD56 800 ; CIK SMMC- 7721B l- 7402H p- 3bH IC- 25 1 70% ~ 80% , , ; L- 02 5% ; CIK K562 K562 70% 结论: CIK , , , [ ] ; CIK ; ; , ; , [ ] R730. 5 [ ] A Studies on Induction of CIK from PBMC with umbilical sera HUANG Ling, ZHANG You- shun, WANG D - shan, et al. (Hep atosurgery lnstitute, Central Hosp ital of Dongf eng Motor Comp any, Shiyan, H ubei China 442000 ) Abstract: Objective T o obs rv if th umbilical s ra could r plac normal AB s ra in th proc ss s that th CIK b induc d from PBMC. Methods Inducing CIK r sp ctiv ly in vitro from PBMC of normal p opl w ith cultur s containing normal A B s ra or umbilical s ra, to find out wh th r th r is any diff r nc b tw n tw o groups of CIK w hil th c lls incr as- 3 ing; d t cting th c ll surfac mark rs CD3 and CD56 of two groups; d t cting cytotox icity of two CIK c lls by H - T dR r l as m thod to diff r nt targ t c lls ( SMMC- 7721, B l- 7402, H p- 3b, all from human liv r canc r, HIC- 251, from int stin canc r, K562, l uka mia, and L- 02, from f tal liv r but not canc r) . Results th numb r of c lls of PBMC incr as d both in normal s ra cultur and in umbilical s r