栀子金花丸中黄芩苷的含量测定_刘元媛.pdfVIP

20 11 A ug132( 4) J L iaon ing M ed ica l U n ive rs ity 357 刘元媛, 卢静华, 郭伟英, 李冰 ( , 12 1000 ) : 建立栀子金花丸中黄芩苷的含量测定方法 采用 H PLC 测定栀子金花丸中黄芩苷的含 量, 色谱条件为H yp ersiL OD S ( 5 Lm, 416 @ 200 mm ) 柱, 以甲醇- 水- 磷 ( 47: 53: 012) 为流动相 此方法线性良好, 平均加样回收率为 10014% , R SD 为 112% 本方法精密度高, 分离度良好, 可用于测 定栀子金花丸中黄芩苷的含量 : 高效液相色谱法 黄芩 黄芩苷 : R 9271 2 : A : 1674 - 0424 ( 2011) 04 - 0357 - 0 3 Determine the ContentofBaicalin inGardenia Camus Pills byHPLC L IU Y u anyuan, LU J inghua, GU O W eiy ing, L I B ing ( L iaoning M edical U niversity, J inzhou 121000 Ch ina) Abstract: Objective T o w o rk out a m ethod to determ ine the content of Ba icalin in G arden ia Camu s P ills. M ethods H PLC w as u sed to de term ine the content of ba ica lin in G arden ia C amu s p ills. The chrom atographic condition w as pe rfo rm ed on D iscovery C 18 co lum n ( 5Lm 416 @ 200 mm ) w ith a m ix ture o f m eth ano l- w ater- phosph ate ( 47: 5 3: 01 2) asm ob ile ph ase. Results T he m ethodps linearity w as sound. T he average recovery w as 10014% , and RSD w as 112% . Conclusion The m ethod prove to be qu ite prec ise and w ell d isso lved, and it is a good m ethod to determ ine ba ica lin in G ardenia Cam u s P ills. eywords: H PLC Scutellaria b a ica lin , 2 , 211 : H yp ersiL OD S ( 5 Lm , 416 @ 200 , mm ) : ) ) ( 47: 5 3: , , 012) [ 1] : 110 mL /m in : 25 e ,