镉对大鼠海马神经元甘氨酸受体的抑制作用.pdfVIP

Inhibitory effect of Cd2+ on glycine receptors in rat hippocampal neurons Wang Shu Ruan Di-Yun * wangshu@ Ruandy@ School of Life Science, University of Science Technology of China, Hefei, Anhui 230027, P.R. China Abstract The effects of cadmium (Cd2+) on glycine-induced Cl － current (IGly) were investigated in acutely dissociated rat hippocampal CA1 neurons using the conventional whole-cell patch-clamp technique in this study. We found that Cd2+ reversibly and concentration-dependently reduced the amplitudes of IGly, with an IC50 of 1.27 mM and Hill coefficient of 0.45. The depression of IGly by Cd2+ was independent of membrane voltage between -60mV and +40mV and didn’t involve a shift in the reversal potential of the current. A noncompetitive inhibition was suggested by a double reciprocal plot of the effects of Cd2+ on the concentration-response curve of the IGly. Since intracellular dialysis with 3 mM Cd2+ failed to modify IGly, it was suggested that the site of action of Cd2+ is extracellular. The suppression of IGly by Zn2+ was unaffected by 3 mM Cd2+, which indicated that Zn2+ and Cd2+ bind to independent sites on glycine receptor. The results show that Cd2+ decreases IGly in acutely dissociated rat hippocampal neurons and the depression of IGly by Cd2+ may contribute to worsen the neurotoxicological impairment. Key Words: Cd2+; glycine receptor; whole-cell recording; hippocampal neuron; neurotoxicity *Corresponding Author: This work was supported by