波棱瓜子提取物对小鼠抗疲劳耐缺氧作用的研究.docVIP

波棱瓜子提取物对小鼠抗疲劳耐缺氧作用的研究 修改稿 靳世英①②, 吕俊兰①②, 袁海龙① ,靳士晓①②，付珊珊①②，郭静静①② [摘 要] 目的 研究波棱瓜子提取物对小鼠抗疲劳耐缺氧的作用。方法 按设定剂量连续灌胃给药7天后，观察小鼠疲劳游泳时间及耐缺氧存活时间，探索波棱瓜子提取物对小鼠抗疲劳耐缺氧的作用。结果 疲劳游泳实验中，复方丹参片组、波棱素高、中、低剂量组疲劳游泳时间与空白对照组比较均有显著性差异（P0.05）；常压耐缺氧实验中，波棱高、中剂量组耐缺氧存活时间与空白组比较有显著性差异（P0.01），而复方丹参片组、波棱低剂量—组与之相比无显著性差异（P0.05）。结论 波棱瓜子提取物能显著提高小鼠抗疲劳耐缺氧能力。 [关键词] 波棱瓜子；抗疲劳；耐缺氧 Studies on Anti-fatigue and Anoxia-resistant effects of Herpetospermum Caudigerum Extracts in mice JIN Shi-ying①②, LV Jun-lan①②, YUAN Hai-long①, JIN Shi-xiao①②, FU Shan-shan①②, GUO Jing-jing①② ①. Department of Pharmacy, 302 Military Hospital of China, Beijing 100039, China； ②. School of Pharmaceutical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611730, China [Abstract] Objective To study the Anti-fatigue and Anoxia-resistant effects of Herpetospermum Caudigerum Extracts(HCE) in mice. Methods 80 mices were administered with drugs on certain doses by gastric perfusion for 7 days, twice daily. One hour after the last administration, swimming method was employed to observe the effect of HCE on fatigue endurance, and swimming time was recorded; The closed hypoxia test was carried out to study the effect of HCE on the hypoxia tolerance in mice. Results In the swimming experiment, the results showed that compound Danshen tablets group and each HCE group had significant effects on fatigue endurance compared to the blank control group (P0.05); There was a notable difference in hypoxia tolerance between HCE groups( high-dose and middle- dose HCE groups) and the blank control group in the oxygen deficiency resistance experiments (P0.05), but compound Danshen and low-HCE groups had no difference compared to the control group. Conclusion These results indicated that HCE had remarkable effects on the increase of fatigue endurance and hypoxia tolerance in mice. [Key words] Herpetospermum Caudigerum; anti-fatigue; anoxia-resistant 藏药波棱瓜子为葫芦科植物波棱瓜Herpetospermum Caudigerum的干燥种子，主产于西藏西南部，尼泊尔和印度东北部，分布的海拔为 2000~ 3500 米。文献记