大球盖菇硒多糖体内抗氧化能力检测.pdf

物技术通报 研究报告 BIOTECHNOLOGY BULLETIN 2009 年增刊 大球盖菇硒多糖体内抗氧化能力检测 1袁2 1 2 2 苗元振 马继波 贾乐 宋振 1 2 ( 泰山学院，泰安 271000 ；山东农业大学生命科学学院，泰安 2710 18) 摘 要： 采用液体发酵的方法对大球盖菇菌丝进行富硒培养，提取硒多糖进行抗氧化能力测定。结果表明，大球盖菇硒 多糖能明显提高小鼠血中SOD、GSH-Px 的含量，能在一定程度上显著降低血中MDA 的含量，具有显著的抗氧化功能。 关键词： 大球盖菇 硒多糖 体内抗氧化 The Antioxidant Activity Determination of Se-polysaccharide from SQ-01 Miao Yuanzhen 1,2 Ma J ibo 1 J ia Le 2 Song Zhen 2 (1 College of Tais han, Taian 271000 曰 2 College of Life Sc ience , Shandong A gricultural Unive rs ity , Taian 271018) Abstract: The objectives of this work were to extract the Se-polysaccharide of Se-enriched mycelia by Stropharia rugoso-annu- lata SQ-01 in liquid media, and to evaluate the antioxidant activities of Se-polysaccharide from S. rugoso-annulata SQ-01 in vivo in terms of the values of glutathione peroxidase (GSH-Px) , superoxide dismutase (SOD) and malondialdehyde (MDA) . The results obtained showed that the Se-polysaccharide of S. rugoso-annulata SQ-01 could obviously elevate GSH-Px and SOD activities and decrease MDA content in mice blood, indicating that the Se-polysaccharide has potentially antioxidant activities. Key words: Stropharia rugoso-annulata SQ-01 Se-polysaccharide Antioxidant activity in vivo 硒渊Selenium 冤是人体必需微量矿质营养元素之 目前国内外尚未见相关报道遥 一 是人体生理功能红细胞谷胱甘肽过氧化物酶和 1 材料与方法 磷脂过氧化氢谷胱甘肽过氧化物酶的重要组成成 1.1 材料 [1] 分 遥 而硒多糖作为一种有机硒化合物兼有多糖和 1.1.1 供试