土壤中降解涕灭威菌株的分离鉴定及降解特性.pdf

农药学学报2007，9(4)：383—389 ChineseJournalPesticideScience of ·研究论文· 土壤中降解涕灭威菌株的分离鉴定及降解特性 冯秀斌， 庞民好， 刘颖超+， 孔俊英 (河北农业大学植物保护学院，河北保定071001) 摘要：为探明土壤微生物对涕灭威的降解能力，用富集培养法分离驯化土壤中涕灭威的优势降解 茵，初步筛选出了对涕灭威具有较高降解能力的菌株TB26和100．8。经过生理生化鉴定和16S rDNA序列同源性分析，将菌株TB26初步鉴定为克雷伯杆菌属(Klebsiellasp．)，菌株100．8初步鉴 定为枯草芽孢杆菌属(Bacillus 氮源分别为蛋白胨、脲。基础无机盐培养基和缺氮培养基对两种茵的生长情况及降解率的影响不 同，外加氮源能够提高100—8的降解率，而缺氮培养基中TB26的降解率较高。 关键词：涕灭威；降解菌；土壤微生物 中图分类号：X172 文献标志码：A 文章编号：1008—7303(2007)04-0383-07 IsolationandIdentificationofSoil of Micro-organismsCapableDegrading AldicarbanditsCharacteristicsof Degradation FENG KONG Xiu—bin，PANGMin—hao，LIUYing—chao+， Jun—ying ofPlantProtection，HebeiAgricultural 071001，HebeiProvince，China) (College University，Baoding Abstract：Forthe ofaldicarb strainsofbacteria exploringdegradationability bymicroorganisms，two as fromsoil enrichmentculture of TB26and isolated capabledegradingaldicarb，named 100．8，were by method．TB26and100．8wereidentifiedasKlebsiellaBacillus biochemical— sp．and sp．respectivelyby sourcefor identificationand ofthe16SrDNA．The carbon physiological sequenceanalysis optimum ofTB26and100．8weremaltobioseandD—fructose source growth respectively，theoptimumnitrogen were andureafor ofTB26and100—8 culturemediumandculture peptone growth respectively．Basic mediumwi