顺铂联合米托蒽醌调节脑胶质瘤u87细胞sonic hedgehog信号通路de研究.pdfVIP

· 1306 · U87细胙 【SonicH 顺铂联合米托蒽醌调节脑胶质瘤 U87细胞 SonicHedgehog信号通路 的研究 尹宜发 ，杨 凡 ，覃晓琳 ，周海波 ，刘朝奇。 EffectofmitoxantronecombinedwithcisplatinonSonicHedgehogsignalpathwayofgli- omacelllineU87 YINYi—fa，YANGFan，QINXiao一1in，ZHOUHai—bo，LIUChao—qi S‘econdPeoplesHospitalofYichang(TumorHospita1)，Yichang443000，China；InstituteofMolecularBiology，ThreeGorgesUniversi— ty，Yichang443002，China ． 【Abstract】 Objecfive：Toobservetheeffectofmitoxantrone(MXT)combinedwithcisplatin(CDDP)onSonic HedgehogsignalpathwayofgliomacelllineU87invitro．M ethods：Thesurvival rateofU87cellsaftertreatmentwith differentconcentrationsofMXT．CDDPandthesameconcentrationsofMXTplusCDDPwereobservedbyMTT as— say．UsingDiOC6dyestainingtodetectthemitochondriamembrna epotentialchangesinU87cellsnadmorphological changeswereobservedhteapoptosis．TheexpressionofGlilandPtchgeneonU87cellsweredetectedbyRT—PCR． Results：MTrandmitochondrialmembranepotentialassayindicatedthatCDDPatlowconcentrations(≤0．625g／ m1)combinedwithMXTcansignificantlyenhancetheinhibitoryeffectandapoptosisofU87cells．RT—PCRassay showedhtatCDDPincreasedhteexpressionofPtchandGlilgene，butMXTandMXT+CDDPcandecreasehtePtch andGlilgeneexpression．Conclusion：U87gliomacellstreatedwiht MXTMoneortreatedwithMXTcombinedwith CDDPcanaffecttheSonicHedgehogsignalingpathway，whichplayedanimportnatroleinenhancingtheapoptosis， thusenhancingthesensitivityofU87cellstochemotherapeuticdrug． K【eywords】glioma；MXT；CDDP；SonicHedgehogsignalpathway ModernOncology2011，19(07)：1306—1309 【摘要 】 目的：观察米托蒽醌(Mitoxantrone，MXT)联合顺铂(Cisplatin，CDDP)对脑胶质瘤U87细胞杀伤活 性及对 SonicHedgehog信号通路的影响。方法：应用MTF法检测不同浓度米托蒽醌、顺铂以及两药物联合对 U87细胞成活率的影响。显微镜观察细胞的形态变化DiOC6荧光染料对细胞线粒体染色检测其膜电位变化 来反映细胞凋亡。RT—PCR法检测顺铂、米托蒽醌及两药联合对U87细胞Glil和Pteh基因表达的影响。结 果：MIq结果显示顺铂、米托蒽醌均可以有效抑制 U87细胞的增殖 ，当米托