化学发光法研究三种参对DNA损伤的保护作用.pdfVIP

The study of anti-oxidation of red ginseng 、shengshai ginseng 、American ginseng and the protective effect on DNA with chemiluminescence Fang Rongmei, He Pingang, Li Xiaojun, Fang Yuzhi (Department of Chemistry, East China Normal Univeristy, Shanghai 200062) Abstract In this paper, weve studied the protective effects of three kinds of ginseng(red ginseng 、shengshai ginseng and American ginseng) on DNA against the oxidative damage in CuSO -Phen-Vc-H O -DNA chemiluminescence 4 2 2 mixture. We find that the protective effect of red ginsengs extract is the best. The protective effect of shengshai ginseng is better than that of American ginseng.The reason is that the amount of effective component(sancun zaodai) in ginseng(including red ginseng and shengshai ginseng) is larger than that in American ginseng, and because of different process, new antioxidants such as maltol and salicylaldehyde are formed in red ginseng. Key words DNA damage, Chemiluminescence, Red ginseng, Shengshai ginseng, American ginseng 摘要利用CuSO -Phen-Vc-H O -DNA化学发光体系研究了红参、生晒参、西洋参对DNA氧化损伤的保护作 4 2 2 用。通过实验发现，对DNA的保护作用为红参优于生晒参、生晒参优于西洋参。因为人参(红参和生晒参)所含 的有效成分三醇皂甙的量比西洋参多,而红参由于加工过程不同，产生新的抗氧化剂如maltol和水杨醛。 关键词 DNA 损伤化学发光红参生晒参西洋参 化学发光法研究三种参对DNA损伤的保护作用* 方荣美何品刚李晓军方禹之** (华东师范大学化学系上海 200062) DNA是生物体内一种重要的大分子,它的氧化损伤在癌症、衰老等疾病的发生过程中起着重要作用。.OH 一 直被认为是引起DNA损伤的重要因素。生命体内的.OH可通过抽提核酸分子中碱基氢或戊糖氢，从而引 起DNA碱基发生开环、脱落等变化，使DNA双螺旋间的氢键遭到破坏，DNA发生单链或双链断链、交联等作 [1] . . - . - 用 。虽然活性自由基除OH外还有O ，一般认为O 对DNA等生物分子的损伤是由于它与H O 作用形成中 2 2 2 2 间产物·OH引起[2] . 。因此，研究抑制OH对DNA损伤的