卡维地洛对缺氧再供氧心肌细胞缝隙连接通道蛋白43表达的影响.docVIP

卡维地洛对缺氧、再供氧心肌细胞缝隙连接通道蛋白43表达的影响 作者:曾玉杰,冯义柏 作者单位：中日友好医院心内科，北京市 100029 【摘要】 目的：检测卡维地洛(Cavedilol，CVD)对缺氧再供氧时心肌细胞缝隙连接通道蛋白43（Conexin 43,CX43）的基因表达和CX43通道蛋白变化的影响。方法：原代培养心肌细胞，随机分为2组：未用药组，CVD组。建立缺氧、再供氧模型，分别于正常、缺氧30 min、再供氧1 h、2 h、3 h、6 h搜集细胞。逆转录聚合酶链式反应（RT-PCR）检测CX43mRNA表达、蛋白免役印迹法（Western blot）检测CX43蛋白的含量。结果：未用药组的心肌细胞缺氧30 min时CX43mRNA表达与蛋白含量和正常相比无显著差异（P005）。再供氧1 h、2 h、3 h、6 h则分别减少了3916%、4500%、4667%、5167%，和正常时相比差异显著（P001）。其中再供氧1 h下降幅度最大。用CVD干预后再供氧1 h、2 h、3 h、6 h则分别减少了2295%、2787%、3033%、3525%（P001）。再供氧1 h、6 h的下降幅较未用药组分别减少了4139%、3178%，差异显著（P001）。结论：心肌细胞缺氧再供氧时，其CX43基因表达和蛋白含量明显减少， 卡维地洛可抑制CX43降解。 【关键词】 卡维地洛 缝隙连接通道蛋白43 卡维地洛 心肌再灌注 Cavedilol influence on CX43 expression in myocardial cells with oxygen defect and resupply/ZENG Yu|jie,FENG Yi|bai,YU Shi|long,ZHU Dan|dan,LI Zhi|yuan,LI Nan,JIANG Lei,WANG Yong,KE Yuan|nan//Chinese Journal of Cardiovascular Rehabilitation Medicine,2007，16（3）：266 Abstract：Objective:To investigate the effects of carvedilol （CVD）on expression of conexin43（CX43） gene and CX43 protein in myocardial cells with oxygen defect and resupply.Methods:A culture system of neonatal cardiac myocytes and the model of the myocardial cells with oxygen defect and resupply were established.The cultured myocytes were randomly divided into two groups: control group(without CVD treated), CVD group.The cells were collected at normal,oxygen defect for 30min,and oxygen resupply for 1h,2h,3h and 6hThe CX43 mRNA level of cells was determined with reverse transcriptase polymerase chain reaction(RT-PCR).The content of protein was detected by western blotting.Results:In control group, there were no significantly different of CX43 expression between normal and myocardial cells oxygen defect for 30 min (P005).The CX43 depression decreased 3916%、4500%、4667%、5167% in myocardial cells oxygen resupply for 1h,2h,3h and 6h(P001).In CVD group, comparing with normal， the expression of CX43 decreased by 2295%、2787%、3033%、3525% in ox